L3MBTL1 antibody (pAb)

for L3MBTL1 antibody (pAb)Background

L3MBTL1 is a human homolog of the Drosophila l(3)mbt protein. Located on chromosome 20q12, L3MBT1 is a member of the malignant brain tumor (MBT) protein family and directly compacts chromatin in a strictly histone methylation dependent fashion. L3MBTL1 functions as a transcriptional repressor in a manner that requires its three MBT domains, which were predicted to bind methylated lysines of histones. L3MBTL1 binds to Lys20 of Histone H4 that has been mono- or dimethylated, as well as to Histone H1 isotype b methylated at lysine 26. L3MBTL1 is a complex molecule with a Zinc-finger and a sterile alpha motif (SAM).

for L3MBTL1 antibody (pAb)Immunogen

This L3MBTL1 antibody was raised against a His-tagged fusion protein corresponding to amino acids 1-215 of human L3MBTL1.

for L3MBTL1 antibody (pAb)Application Notes

This L3MBTL1 antibody has been validated for use in Western blotting (1:500 - 1:2,000), and has been reported to work in chromatin IP and immunoprecipitation. While an 86 kDa band is predicted, a ~100 kDa band was observed. Slight reactivity of a 70 kDa protein is also observed.

for L3MBTL1 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for L3MBTL1 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for L3MBTL1 antibody.

for L3MBTL1 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for L3MBTL1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.