| Antibody Type: | Monoclonal |
| Isotype: | Mouse IgG1k |
| Purification: | Ascites |
|
| Appl.: | IF |
| Reactivity: | Human, Mouse |
| Molecular Weight: | 370 kDa |
|
| Western Blot |
Chromatin IP |
Immunofluorescence |
| Not tested |
Not tested |
1:500 |
Background for ATM phospho Ser1981 antibody (mAb) (Clone 10H11.E.12):
ATM –
Ataxia Telangiectasia Mutated is a PI3-family protein kinase and a critical cell cycle checkpoint protein. ATM and a related protein, ATR, play crucial roles in the maintenance of genome integrity and the response to DNA damage. Upon activation, ATM phosphorylates itself at serine 1981 and then phosphorylates a number of downstream proteins (
e.g. BRCA1,
CHK2,
H2AX,
p53,
NBS1, SMC1) resulting in cell cycle arrest and the initiation of DNA damage repair. Loss of function of ATM is causal to a variety of syndromes involving increased incidence of several cancers.
Immunogen for ATM phospho Ser1981 antibody (mAb) (Clone 10H11.E.12):
This antibody was raised against a peptide containing phospho-serine 1981 of human ATM.
Application Notes for ATM phospho Ser1981 antibody (mAb) (Clone 10H11.E.12):
This ATM phospho Ser1981 antibody (Clone 10H11.E.12) has been validated for use in immunofluorescence (1:500 dilution) on HeLa cells treated with gamma irradiation to cause double strand DNA breaks.
Buffer:
Ascites containing 30% glycerol and 0.035% sodium azide.
Positive Control:
Active Motif's
HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for ATM phospho Ser1981 antibody (Clone 10H11.E.12).
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
References:
This antibody was used in Bakkenist and Kastan (2003)
Nature 421:499.
ATM phospho Ser1981 antibody (Clone 10H11.E.12) tested by immunofluorescence.
HeLa cells stained with ATM phospho Ser1981 antibody (Clone 10H11.E.12) at a 1:500 dilution using using MAX Stain™ Immunofluorescence Tools.
Panel A: Normal HeLa cells.
Panel B: HeLa cells treated with 3 Gy ionizing radiation and collected 30 minutes post-exposure.
Top images: Cells stained with DAPI.
Middle images: Same cells stained with ATM phospho Ser1981 mAb (Clone 10H11.E.12).
Bottom images: Merge of the two images above.
Note the Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of ATM at serine 1981. In contrast, Panel A-middle image shows no detectable phosphorylated ATM.
Storage:
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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