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STAT3 phospho Tyr705 antibody (pAb)
| Catalog No: 39595 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39596 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for STAT3 phospho Tyr705 antibody (pAb)Background
STAT3 (signal transducer and activator of transcription 3) is a member of the STAT family of transcription factors. STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNα, IL-10, IL-6, IL-11, IL-12, IL-2, EGF, among others.
for STAT3 phospho Tyr705 antibody (pAb)Immunogen
This STAT3 phospho Tyr705 antibody was raised against a peptide containing phosphorylated Tyr705 of human STAT3.
for STAT3 phospho Tyr705 antibody (pAb)Application Notes
This STAT3 phospho Tyr705 antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution).
for STAT3 phospho Tyr705 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol, 0.035% sodium azide.
for STAT3 phospho Tyr705 antibody (pAb)Positive Control
Active Motif's Hep G2 nuclear extract (IL-6 stimulated, 100 ng/ml) (Catalog No. 36107) can be used as a positive control for STAT3 phospho Tyr705 antibody.
STAT3 phospho Tyr705 pAb tested by Western blot.
Hep G2 nuclear extract (15 µg per lane) was probed with STAT3 phospho Tyr705 polyclonal antibody (1:2,000).
Lane 1: No treatment.
Lane 2: cells stimulated with IL-6 (100 ng/ml for 15 minutes at 37°C).
STAT3 phospho Tyr705 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of STAT3 phospho Tyr705 pAb for STAT3 phospho Tyr705. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: Unmodified Ser727 STAT1 peptide.
Lane 2: Phospho Ser727 STAT1 peptide.
Lane 3: Unmodified Tyr689 STAT2 peptide.
Lane 4: Phospho Tyr689 STAT2 peptide.
Lane 5: Unmodified Ser727 STAT3 peptide.
Lane 6: Phospho Ser727 STAT3 peptide.
Lane 7: Unmodified Tyr705 STAT3 peptide.
Lane 8: Phospho Tyr705 STAT3 peptide.
Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide.
Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide.
Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide.
Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide.
for STAT3 phospho Tyr705 antibody (pAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for STAT3 phospho Tyr705 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
