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Antibodies

Aiolos antibody (mAb)

Clone: 8B2
Aliases: IKZF3, AIO, ZNFN1A3
Catalog No: 39657 Format: 100 µg $410 Buy Now
Catalog No: 39658 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:70 kDa
Reactivity:Mouse

Applications

ChIP Validated Western Blot Validated

for Aiolos antibody (mAb) (Clone 8B2)Application Notes


Published Applications:
ChIP
WB
See references for more information. Individual optimization may be required.

for Aiolos antibody (mAb) (Clone 8B2)Immunogen

This Aiolos antibody was raised against the N-terminal half of mouse Aiolos protein.

for Aiolos antibody (mAb) (Clone 8B2)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Aiolos antibody (mAb) (Clone 8B2)References

for Aiolos antibody (mAb) (Clone 8B2)Background

Aiolos (also known as IKZF3, Ikaros family zinc finger 3 and ZNFN1A3) belongs to a family of zinc-finger proteins that also include Ikaros and Helios. Aiolos is a transcription factor that is important in the regulation of lymphocyte proliferation and differentiation, which is preferentially expressed in peripheral blood leukocytes, spleen and thymus tissue. Both Ikaros and Aiolos can also participate in chromatin remodeling.

for Aiolos antibody (mAb) (Clone 8B2)Positive Control

This antibody can be used with lymphocytes, spleen, thymus or bone marrow samples.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Aiolos antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Aiolos antibody (mAb).

for Aiolos antibody (mAb) (Clone 8B2)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Aiolos antibody (mAb) (Clone 8B2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Aiolos antibody (mAb)