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Antibodies

NFκB p100 antibody (pAb)

Aliases: p52, p105, H2TF1, LYT10, CVID10, LYT-10, NF-kB2
Catalog No: 39687 Format: 100 µl $410 Buy Now
Catalog No: 39688 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:105 kDa
Reactivity:Human

Applications

Western Blot Validated

for NFkB p100 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for NFkB p100 antibody (pAb)Immunogen

This NFkB p100 antibody was raised against a peptide derived from the C-terminal region of human NFkB p100.

for NFkB p100 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NFkB p100 antibody (pAb)References

 
NFκB p100 antibody (pAb) tested by Western blot.

NFκB p100 pAb tested by Western blot.
Detection of NFκB p100 by Western blot analysis. NFκB p100 is detected in nuclear extracts derived from Jurkat cells using NFκB p100 rabbit polyclonal antibody at a 1:2,000 dilution.

for NFkB p100 antibody (pAb)Background

NFκB p100 (Nuclear factor NF-kappa-B p100 subunit also known as NFκB2 p100) is a member of the NFkB family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The family also includes c-Rel, RelA (NFκB p65, NFκB3) and RelB (I-Rel). Rel proteins form dimers with the NFκB p50 (proteolytically processed from p105/NFκB1) and p52 (proteolytically processed from p100/NFκB2) members of the NFκB family that bind DNA and activate transcription. The NFκB p65/p50 heterodimer is the most abundant form of NFκB.

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB. The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ). Upon its activation, the IKK complex phosphorylates IκB proteins (IκBα, IκBα phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFκB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

for NFkB p100 antibody (pAb)Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for NFκB p100 pAb.

for NFkB p100 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NFkB p100 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NFκB p100 antibody (pAb)
 

Data Thumbnails

NFκB p100 antibody (pAb) tested by Western blot.

Western blot of NFκB p100 pAb.
(Click image to enlarge and see details.)