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NFκB p65 phospho Ser529 antibody (pAb)
| Catalog No: 39691 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39692 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:1,000 | — |
for NFkB p65 phospho Ser529 antibody (pAb)Background
NFκB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFκB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFκB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFκB p50 (proteolytically processed from p105/NFκB1)and p52 (proteolytically processed from p100/NFκB2) members of the NFκB family that bind DNA and activate transcription. The NFκB p65/p50 heterodimer is the most abundant form of NFκB.
NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB. The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ). Upon its activation, the IKK complex phosphorylates IκB proteins (IκBα, IκBα phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFκB complex to translocate to the nucleus where it regulates transcription by binding to DNA.
for NFkB p65 phospho Ser529 antibody (pAb)Immunogen
This NFkB p65 phospho Ser529 antibody was raised against a peptide containing phospho-serine 529 of human NFkB p65.
for NFkB p65 phospho Ser529 antibody (pAb)Application Notes
This product has been validated for use in Western blotting (1:500 - 1:1,000 dilution).
for NFkB p65 phospho Ser529 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
NFkB p65 phospho Ser529 pAb tested by Western blot.
HeLa cell extract (20 µg per lane) probed with either NFkB p65 phospho Ser529 pAb (1:1,000 dilution. Lane 1: extract derived from untreated cells. Lane 2: extract derived from cells treated with TNF-alpha and Calyculin.
NFkB p65 phospho Ser529 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of of NFkB p65 phospho Ser529 pAb for NFkB p65 phosphorylated at serine 529. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with NFkB p65 phospho Ser529 pAb at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: phospho Ser276 NFkB p65 peptide.
Lane 2: unmodified peptide surrounding Ser276 NFkB p65 peptide.
Lane 3: phospho Ser529 NFkB p65 peptide.
Lane 4: unmodified peptide surrounding Ser529 NFkB p65.
Lane 5: phospho Ser536 NFkB p65 peptide.
Lane 6: unmodified peptide surrounding Ser536 NFkB p65.
Lane 7: phospho Ser337 NFkB p50 peptide.
Lane 8: unmodified peptide surrounding Ser337 NFkB p50.
for NFkB p65 phospho Ser529 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for NFkB p65 phospho Ser529 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
