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Antibodies

NFκB p65 phospho Ser529 antibody (pAb)

Aliases: RELA, p65, NFKB3
Catalog No: 39691 Format: 100 µl $410 Buy Now
Catalog No: 39692 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:65 kDa
Reactivity:Human

Applications

Western Blot Validated

for NFkB p65 phospho Ser529 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for NFkB p65 phospho Ser529 antibody (pAb)Immunogen

This NFkB p65 phospho Ser529 antibody was raised against a peptide containing phospho-serine 529 of human NFkB p65.

for NFkB p65 phospho Ser529 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NFkB p65 phospho Ser529 antibody (pAb)References

 
NFκB p65 phospho Ser529 antibody (pAb) tested by Western blot.

NFκB p65 phospho Ser529 pAb tested by Western blot.
HeLa cell extract (20 µg per lane) probed with either NFκB p65 phospho Ser529 pAb (1:1,000 dilution. Lane 1: extract derived from untreated cells. Lane 2: extract derived from cells treated with TNF-α and Calyculin.

NFκB p65 phospho Ser529 antibody (pAb) tested by dot blot analysis.

NFκB p65 phospho Ser529 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of of NFκB p65 phospho Ser529 pAb for NFκB p65 phosphorylated at serine 529. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with NFκB p65 phospho Ser529 pAb at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: phospho Ser276 NFκB p65 peptide. Lane 2: unmodified peptide surrounding Ser276 NFκB p65 peptide. Lane 3: phospho Ser529 NFκB p65 peptide. Lane 4: unmodified peptide surrounding Ser529 NFκB p65. Lane 5: phospho Ser536 NFκB p65 peptide. Lane 6: unmodified peptide surrounding Ser536 NFκB p65. Lane 7: phospho Ser337 NFκB p50 peptide. Lane 8: unmodified peptide surrounding Ser337 NFκB p50.

for NFkB p65 phospho Ser529 antibody (pAb)Background

NFκB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFκB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFκB p65, NFκB3) and RelB (I-Rel). Rel proteins form dimers with the NFκB p50 (proteolytically processed from p105/NFκB1)and p52 (proteolytically processed from p100/NFκB2) members of the NFκB family that bind DNA and activate transcription. The NFκB p65/p50 heterodimer is the most abundant form of NFκB.

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB. The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ). Upon its activation, the IKK complex phosphorylates IκB proteins (IκBα, IκBα phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFκB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

for NFkB p65 phospho Ser529 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (TNF-α stimulated) (Catalog No. 40210) can be used as a positive control for NFκB p65 phospho Ser529 antibody.

for NFkB p65 phospho Ser529 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NFkB p65 phospho Ser529 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NFκB p65 phospho Ser529 antibody (pAb)
 

Data Thumbnails

NFκB p65 phospho Ser529 antibody (pAb) tested by Western blot.

Western blot of NFκB p65 phospho Ser529 pAb.
(Click image to enlarge and see details.)

NFκB p65 phospho Ser529 antibody (pAb) tested by dot blot analysis.

Dot blot of NFκB p65 phospho Ser529 pAb.
(Click image to enlarge and see details.)