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Antibodies

CAF-1 p60 antibody (mAb)

Clone: 24
Aliases: CAF1, MPP7, CAF-1, CAF1A, CAF1P60, CAF-IP60, MPHOSPH7
Catalog No: 39995 Format: 100 µg $410 Buy Now
Catalog No: 39996 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:60 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

for CAF-1 p60 antibody (mAb) (Clone 24)Application Notes


Validated Applications:
ICC/IF: 1:2,000 dilution

Published Applications:
IP
ICC/IF
WB
See references for more information. Individual optimization may be required.

for CAF-1 p60 antibody (mAb) (Clone 24)Immunogen

The CAF-1 p60 antobody was raised against full-length CAF-1 purified from human 293 cells.

for CAF-1 p60 antibody (mAb) (Clone 24)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CAF-1 p60 antibody (mAb) (Clone 24)References

 
CAF-1 p60 antibody (mAb) tested by immunofluorescence.

CAF-1 p60 antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with CAF-1 p60 antibody at a 1:2,000 dilution.

CAF-1 p60 antibody (mAb) tested by Western blot.

CAF-1 p60 antibody (mAb) tested by Western blot.
CAF-1 p60 (mAb) detection by Western blot. The analysis was performed using WT mouse testis and the CAF-1 p60 (mAb) at a 1 µg/ml dilution.

for CAF-1 p60 antibody (mAb) (Clone 24)Background

CAF-1 p60 (Chromatin assembly factor-1 p60 subunit, CAF1B, CHAF1B) is a subunit of the CAF-1 complex that is required for the assembly of nucleosomes onto newly replicated DNA. Specifically, CAF-1 assists in the loading of the H3-H4 tetramer and may be involved in DNA damage repair. CAF-1 p60 is a WD-repeat containing protein related to HIRA.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use CAF-1 p60 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CAF-1 p60 antibody (mAb).

for CAF-1 p60 antibody (mAb) (Clone 24)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CAF-1 p60 antibody (mAb) (Clone 24)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CAF-1 p60 antibody (mAb)
 

Data Thumbnails

CAF-1 p60 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of CAF-1 p60 mAb.
(Click image to enlarge and see details.)

CAF-1 p60 antibody (mAb) tested by Western blot.

Western blot of CAF-1 p60 mAb.
(Click image to enlarge and see details.)