ETO / RUNX1T1 antibody (pAb)

for ETO / RUNX1T1 antibody (pAb)Background

ETO / RUNX1T1 (AML1T1, CBFA2T1, MTG8, ZMYND2) belongs to a family of evolutionarily conserved nuclear factors that act as a transcriptional corepressor. ETO / RUNX1T1 does not interact directly with DNA but is recruited by transcription factors such as PLZF, BCL6, TAL1/SCL, Gfi1and Heb recruit nuclear co-repressors such as NCoR, SIN3, and SMRT / NCoR2, which in turn interact with histone deacetylase (HDAC) to mediate transcriptional repression. ETO / RUNX1T1 contains four evolutionarily conserved functional domains called nervy homology regions (NHRs) involved in protein-protein interaction. ETO / RUNX1T1 is frequently found as a fusion partner of AML1 in acute myeloid leukemia with the t(8;21) translocation which gives rise to the AML-ETO fusion protein.

for ETO / RUNX1T1 antibody (pAb)Immunogen

This ETO / RUNX1T1 antibody was raised against a peptide within the internal region of human ETO / RUNX1T1.

for ETO / RUNX1T1 antibody (pAb)Application Notes

This ETO / RUNX1T1antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution).

For optimal results, primary antibody incubations should be performed overnight at 4°C. Individual optimization may be required.

for ETO / RUNX1T1 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for ETO / RUNX1T1 antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for ETO / RUNX1T1 antibody.

for ETO / RUNX1T1 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for ETO / RUNX1T1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of arrival.