Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

CtIP antibody (mAb)

Clone: 14-1
Aliases: RBBP8, RIM, COM1, JWDS, SAE2, SCKL2
Catalog No: 61141 Format: 100 µg $410 Buy Now
Catalog No: 61142 Format: 10 µg $95 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:120 kDa
Reactivity:Human

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

for CtIP antibody (mAb) (Clone 14-1)Application Notes


Published Applications:
IP
ICC/IF
WB
See references for more information. Individual optimization may be required.

for CtIP antibody (mAb) (Clone 14-1)Immunogen

This CtIP antibody was raised against a recombinant protein corresponding to amino acid residues 620-897 of human CtIP.

for CtIP antibody (mAb) (Clone 14-1)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CtIP antibody (mAb) (Clone 14-1)References

for CtIP antibody (mAb) (Clone 14-1)Background

CtIP (CTBP interacting protein, RBBP8, RIM, COM1) is a transcriptional regulator and tumor suppressor first identified through its interaction with CTBP (C-Terminal Binding Protein). CtIP binds to the Rb protein and serves as a transcriptional repressor, through recruitment of CtBP and also by interaction with general transcription factors TFIID and TFIIB. CtIP also interacts with Rb-related protein p130 and Ikaros, a master regulator in lymphocyte development. CtIP is phosphorylated upon DNA damage (likely by ATM and/or ATR), interacts with BRCA1 and plays a critical role in facilitating DNA damage repair. Mutations that abolish the interaction between CtIP and BRCA1 result in loss of cell cycle control leading to the development of cancer.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use CtIP antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CtIP antibody (mAb).

for CtIP antibody (mAb) (Clone 14-1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CtIP antibody (mAb) (Clone 14-1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CtIP antibody (mAb)