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Antibodies

CtIP antibody (mAb)

Clone: 14-1
Catalog No: 61141 Format: 100 µg $340 Buy Now
Catalog No: 61142 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG1
Purification:Protein G Chromatography
Applications:IF, IP, WB
Reactivity:Human
Molecular Weight:120 kDa

Applications Table

Western Blot Chromatin IP IF IP
Published.
See Application Notes below.		 — Published.
See Application Notes below.		 Published.
See Application Notes below.

for CtIP antibody (mAb) (Clone 14-1)Background

CtIP (CTBP interacting protein, RBBP8, RIM, COM1) is a transcriptional regulator and tumor suppressor first identified through its interaction with CTBP (C-Terminal Binding Protein). CtIP binds to the Rb protein and serves as a transcriptional repressor, through recruitment of CtBP and also by interaction with general transcription factors TFIID and TFIIB. CtIP also interacts with Rb-related protein p130 and Ikaros, a master regulator in lymphocyte development. CtIP is phosphorylated upon DNA damage (likely by ATM and/or ATR), interacts with BRCA1 and plays a critical role in facilitating DNA damage repair. Mutations that abolish the interaction between CtIP and BRCA1 result in loss of cell cycle control leading to the development of cancer.

for CtIP antibody (mAb) (Clone 14-1)Immunogen

This CtIP antibody was raised against a recombinant protein corresponding to amino acid residues 620-897 of human CtIP.

for CtIP antibody (mAb) (Clone 14-1)Application Notes

This CtIP antibody has been validated for use in immunofluorescence, immunoprecipitation and Western blot. See References for more information on specific applications.

for CtIP antibody (mAb) (Clone 14-1)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use CtIP antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CtIP antibody (mAb).

for CtIP antibody (mAb) (Clone 14-1)References

This antibody has been used in the following references:
IF: Zhang Y and Jasin M (20011) Nat Struct Mol Biol 16: 80-85.
IP: Xu Y et al (2006) Genes Dev 20: 1721-1726.
WB: Xu Y et al (2006) Genes Dev 20: 1721-1726.

for CtIP antibody (mAb) (Clone 14-1)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for CtIP antibody (mAb) (Clone 14-1)Guarantee

This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting