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Antibodies

SMARCA4 antibody (mAb)

Clone: 5B7
Aliases: SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 4, BRG1-Associated Factor 190A, BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
Catalog No: 61625 Format: 100 µg $410 Buy Now
Catalog No: 61626 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:180 kDa
Reactivity:Human, Monkey, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for SMARCA4 antibody (mAb) (Clone 5B7)Application Notes


Validated Applications:
ICC/IF: 2 - 10 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

Published Applications:
ICC/IF
See references for more information. Individual optimization may be required.

for SMARCA4 antibody (mAb) (Clone 5B7)Immunogen

This antibody was raised against a recombinant protein corresponding to amino acids 213-295 of human SMARCA4.

for SMARCA4 antibody (mAb) (Clone 5B7)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SMARCA4 antibody (mAb) (Clone 5B7)References

 
SMARCA4 antibody (mAb) tested by immunofluorescence.

SMARCA4 antibody (mAb) (Clone 5B7) tested by immunofluorescence.
Top: HeLa cells stained with SMARCA4 antibody (mAb). Bottom: Hoechst staining.

SMARCA4 antibody (mAb) tested by Western blot.

SMARCA4 antibody (mAb) (Clone 5B7) tested by Western blot.
Whole cell extracts were probed with SMARCA4 antibody (mAb).
Lane 1: L929 cells.
Lane 2: HeLa cells.
Lane 3: Cos cells

for SMARCA4 antibody (mAb) (Clone 5B7)Background

SMARCA4 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4) is the central catalytic ATPase subunit of numerous chromatin-remodeling complexes, including SWI/SNF. These complexes are able to use energy from ATP hydrolysis to physically disrupt chromatin architecture of target promoters and facilitate the binding of transcription factors to nucleosomal DNA. SMARCA4 is a co-regulator of transcription and has been implicated in the activation and repression of gene expression through the modulation of chromatin in various tissues and physiological conditions. This protein plays a major role in many cellular processes such as DNA replication, repair and recombination. Mammalian SMARCA4 is usually associated with approximately 10-12 BAF subunits or other proteins involved in regulation of gene expression. SMARCA4 is part of the stem cell-specific BAF complex, esBAF, and is involved in regulating stem cell pluripotency.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with SMARCA4 antibody (mAb).

for SMARCA4 antibody (mAb) (Clone 5B7)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SMARCA4 antibody (mAb) (Clone 5B7)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SMARCA4 antibody (mAb)
 

Data Thumbnails

SMARCA4 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of SMARCA4 mAb.
(Click image to enlarge and see details.)

SMARCA4 antibody (mAb) tested by Western blot.

Western blot of SMARCA4 mAb.
(Click image to enlarge and see details.)