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Recombinant Proteins & Enzymes

Recombinant SUV420H1 (2-387) protein

Catalog No: 81021 Format: 50 µg $395 Buy Now
Catalog No: 81721 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SUV420H1 (2-387) proteinContents

Recombinant SUV420H1 (2-387) protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SUV420H1 (2-387) proteinBackground

SUV420H1 (Suppressor Of Variegation 4-20 Homolog 1, also known as KMT5B) is a histone methyltransferase that specifically trimethylates nucleosomal histone H4 on lysine 20 (H4K20). H4K20me3 is a histone marker that represents epigenetic transcriptional repression. SUV420H1 mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins. It plays a role in myogenesis by regulating the expression of target genes, such as EID3.

for Recombinant SUV420H1 (2-387) proteinApplication Notes

Recombinant SUV420H1 (2-387) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 2 µg Recombinant Nucleosomes were incubated with different concentrations of SUV420H1 (2- 387) protein in 30 µl reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, and 50 µM SAM for 3 hours at room temperature. Western Blot was used to detect the generation of reaction products.

for Recombinant SUV420H1 (2-387) proteinProtein Details

Recombinant SUV420H1 (2-387) protein corresponding to amino acids 2-387 of SUV420H1 protein (accession number NP_060105.3) was expressed in E. coli cells and contains an N-terminal GST tag with a molecular weight of 71.1 kDa. Recombinant SUV420H1 (2-387) protein contains the SET domain of SUV420H1 that is responsible for methyltransferase activity.

 
Recombinant SUV420H1 (2-387) protein gel.

Recombinant SUV420H1 (2-387) protein gel.
10% SDS-PAGE gel stained with Coomassie blue.
MW: 71.1 kDa
Purity: > 75%

Recombinant SUV420H1 (2-387) activity assay

Recombinant SUV420H1 (2-387) activity assay
2 µg Recombinant Nucleosomes (H3.1) (Cat. No. 31466) were incubated with 0 µg (-), 0.2 µg (+), 0.4 µg (++), 0.8 µg (+++) SUV420H1 (2-387) protein, respectively, in 30 µl reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM for 3 hr at room temperature. 6 µl reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (H4K20me3 antibody, Cat. No. 39672, 1:1000 dilution). SUV420H1 (2-387) only was used as negative control.

The Western blot result shows that nucleosomes are much better than histone octamers as the substrate of SUV420H1 (2-387).

for Recombinant SUV420H1 (2-387) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SUV420H1 (2-387) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant SUV420H1 (2-387) protein
 

Data Thumbnails

Recombinant SUV420H1 (2-387) protein gel.

Recombinant SUV420H1 (2-387) protein gel.
(Click to enlarge and view details)

Recombinant SUV420H1 (2-387) activity assay

Recombinant SUV420H1 (2-387) activity assay
(Click to enlarge and view details).