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Gene Regulation

LightSwitch™ lncRNA Promoter Products

study the regulation of lncRNA promoters

Long noncoding RNAs (lncRNAs) have been shown to play important roles in the regulation of both transcription and chromatin organization. But, the promoters of lncRNA genes appear to be regulated differently than those of protein-coding genes. The LightSwitch Luciferase Assay System makes it fast & easy to study lncRNA promoter regulation using the same materials and methods that have been so successful and popular in studying the regulation of protein-coding gene promoters and miRNA-3´UTR interactions.

IMPORTANT:  Because all LightSwitch reporter constructs contain the optimized RenSP luciferase gene, you MUST use our LightSwitch Luciferase Assay Reagents to obtain optimal results. (Other luciferase assay reagents are not compatible with RenSP.)

You will also need to use an appropriate LightSwitch Promoter Control Vector (such as ACTB or GAPDH) in order to normalize the luciferase signals produced by the lncRNA Promoter Reporter construct when subjected to your chosen stimulation conditions.

 
Name Format Product Info Cat No. Price  
LightSwitch™ FIRRE Promoter Reporter Vector 5 µg Get Info 32071 $475 Buy Now
LightSwitch™ GAS5 Promoter Reporter Vector 5 µg Get Info 32072 $475 Buy Now
LightSwitch™ HOTAIR Promoter Reporter Vector 5 µg Get Info 32073 $475 Buy Now
LightSwitch™ MALAT1 Promoter Reporter Vector 5 µg Get Info 32074 $475 Buy Now
LightSwitch™ MEG3 Promoter Reporter Vector 5 µg Get Info 32075 $475 Buy Now
LightSwitch™ TUG1 Promoter Reporter Vector 5 µg Get Info 32076 $475 Buy Now

Measure lncRNA promoter activity to:

  • Understand the mechanisms by which the expression of a lncRNA gene is induced or repressed
  • Determine the effect of sequence variations on lncRNA promoter function
  • Verify computational predictions and supplement microarray or Next-Generation sequencing data

LightSwitch lncRNA promoter assays

LightSwitch lncRNA Promoter Reporter constructs are made by cloning predicted lncRNA promoter sequences into the pLightSwitch_Prom reporter vector, upstream of the novel, engineered RenSP luciferase gene. After transfection into mammalian cells the samples can be treated, if desired, using whatever condition is to be tested. The luciferase activity is then assayed using the optimized regents found in LightSwitch Luciferase Assay Kit.

For your convenience, we have cloned a number of widely studied lncRNA promoters into the LightSwitch Promoter Reporter vector. These are available as sequence-verified, transfection-ready reporter constructs, so you can begin assays to study how these promoters are regulated immediately, without the need to clone, sequence or prepare the DNA. Alternatively, you can clone your own sequence into an Empty LightSwitch Promoter Reporter Vector or utilize our economical Custom Cloning services.

Diagram detailing the LightSwitch lncRNA Promoter Reporter Assay
Graph showing lncRNA promoter activity of FIRRE, GAS5, MALAT1 and GAS5 in response to paclitaxel stimulation
Figure 1: Measuring the activity of lncRNA promoters.

The promoters of 4 different lncRNA genes were cloned into LightSwitch Promoter Reporter vectors and transfected into MCF-7 cells in triplicate. Following stimulation with DMSO or paclitaxel, luciferase assays were performed using the LightSwitch Luciferase Assay Kit, and the data was normalized by dividing the average of each lncRNA promoter signal by those of a positive control LightSwitch ACTB promoter construct that was assayed under the same conditions.