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Gene Regulation

LightSwitch™ Synthetic Response Elements

the largest collection of synthetic response element reporters

LightSwitch™ Synthetic Response Element reporter constructs simplify the task of determining the effect that test compounds and/or conditions have on over 90 different transcription factors. Each synthRE vector contains an optimized synthetic response element, made up of repeats of a transcription factor binding site motif, cloned upstream of a minimal promoter and the RenSP luciferase gene in the Long-range Enhancer Reporter Vector, pLightSwitch_LR vector. As Synthetic Response Element vectors are supplied transfection-ready, you can begin your experiments immediately.

Similarly, for researchers studying post-transcriptional regulation events mediated by miRNA-3´UTR interactions, we offer a large collection of optimized, synthetic LightSwitch miRNA Target Reporters.

IMPORTANT:  Because all LightSwitch reporter constructs contain the optimized RenSP luciferase gene, you MUST use our LightSwitch Luciferase Assay Reagents to obtain optimal results. (Other luciferase assay reagents are not compatible with RenSP.)

 
Name Pathway Product Info Format Cat No. Price  
AARE synthRE Amino Acid Deprivation Get Info 5 µg 32101 $425 Buy Now
AFP1 synthRE Hepatoma Nuclear Factor Get Info 5 µg 32102 $425 Buy Now
AhR synthRE Xenobiotic/Dioxin Get Info 5 µg 32103 $425 Buy Now
AP-1 synthRE v1 SAPK/JNK Get Info 5 µg 32104 $425 Buy Now
AP-1 synthRE v2 PKC/MAPK Get Info 5 µg 32105 $425 Buy Now
AP-3 synthRE Activator Protein 3 Get Info 5 µg 32106 $425 Buy Now
AR synthRE Androgen Receptor Get Info 5 µg 32107 $425 Buy Now
ARE synthRE Antioxidant Reponse Get Info 5 µg 32108 $425 Buy Now
AREB6 synthRE AREB6 Get Info 5 µg 32109 $425 Buy Now
ATF-1 synthRE cAMP/PKA Get Info 5 µg 32110 $425 Buy Now
ATF-6 synthRE Estrogen Receptor Stress Response Get Info 5 µg 32111 $425 Buy Now
BMP synthRE v1 Bone Morphogenesis Get Info 5 µg 32112 $425 Buy Now
BMP synthRE v2 Bone Morphogenesis Get Info 5 µg 32113 $425 Buy Now
BMP synthRE v3 Bone Morphogenesis Get Info 5 µg 32114 $425 Buy Now
C/EBP alpha synthRE C/EBP Alpha Get Info 5 µg 32115 $425 Buy Now
C/EBP beta synthRE C/EBP Beta Get Info 5 µg 32116 $425 Buy Now
CREB synthRE cyclic AMP Get Info 5 µg 32117 $425 Buy Now
DBP synthRE Insulin Get Info 5 µg 32119 $425 Buy Now
E2F synthRE v1 Cell Cycle Get Info 5 µg 32120 $425 Buy Now
E2F synthRE v2 Cell Cycle Get Info 5 µg 32121 $425 Buy Now
Egr-1 synthRE Early Growth Response Get Info 5 µg 32122 $425 Buy Now
Elk-1 synthRE v1 MAPK/ERK Get Info 5 µg 32123 $425 Buy Now
Elk-1 synthRE v2 MAPK Get Info 5 µg 32124 $425 Buy Now
ER synthRE Estrogen Receptor Get Info 5 µg 32125 $425 Buy Now
ER Stress Response synthRE Estrogen Receptor Stress Response Get Info 5 µg 32126 $425 Buy Now
ERR alpha synthRE v1 Estrogen Receptor Related Receptor Alpha Get Info 5 µg 32127 $425 Buy Now
ERR alpha synthRE v2 Estrogen Receptor Related Receptor Alpha Get Info 5 µg 32128 $425 Buy Now
ERR alpha synthRE v3 Estrogen Receptor Related Receptor Alpha Get Info 5 µg 32129 $425 Buy Now
ERR beta synthRE Estrogen Receptor Related Receptor Beta Get Info 5 µg 32130 $425 Buy Now
FOXO synthRE PI3K/AKT Get Info 5 µg 32131 $425 Buy Now
FXR synthRE v1 Farnesoid X Receptor Get Info 5 µg 32132 $425 Buy Now
FXR synthRE v2 Farnesoid X Receptor Get Info 5 µg 32133 $425 Buy Now
GATA synthRE v1 Erythroid Development Get Info 5 µg 32134 $425 Buy Now
GATA synthRE v2 Erythroid Development Get Info 5 µg 32135 $425 Buy Now
GCNF synthRE v1 Germ Cell Nuclear Factor Get Info 5 µg 32136 $425 Buy Now
GCNF synthRE v2 Germ Cell Nuclear Factor Get Info 5 µg 32137 $425 Buy Now
GCNF synthRE v3 Germ Cell Nuclear Factor Get Info 5 µg 32138 $425 Buy Now
GLI synthRE Hedgehog Get Info 5 µg 32139 $425 Buy Now
GR synthRE Glucocorticoid Receptor Get Info 5 µg 32140 $425 Buy Now
HIF-1 alpha synthRE Hypoxia Get Info 5 µg 32141 $425 Buy Now
HNF-1 synthRE Hepatocyte Nuclear Factor 1 Get Info 5 µg 32142 $425 Buy Now
HNF-4 alpha synthRE Hepatocyte Nuclear Factor 4 Get Info 5 µg 32143 $425 Buy Now
HSF synthRE Heat Shock Get Info 5 µg 32144 $425 Buy Now
IRF-1 synthRE Interferon Regulation Get Info 5 µg 32145 $425 Buy Now
ISRE synthRE JAK/STAT1-2 Get Info 5 µg 32146 $425 Buy Now
KLF4 synthRE KLF4 Get Info 5 µg 32147 $425 Buy Now
LXR alpha synthRE Liver X Receptor Get Info 5 µg 32148 $425 Buy Now
LXRE synthRE v1 Liver X Receptor Get Info 5 µg 32149 $425 Buy Now
LXRE synthRE v2 Liver X Receptor Get Info 5 µg 32150 $425 Buy Now
LXRE synthRE v3 Liver X Receptor Get Info 5 µg 32151 $425 Buy Now
MEF1 synthRE Myocyte Enhancer Factor 1 Get Info 5 µg 32152 $425 Buy Now
MEF2 synthRE Myocyte Enhancer Factor 2 Get Info 5 µg 32153 $425 Buy Now
MEF3 synthRE Myocyte Enhancer Factor 3 Get Info 5 µg 32154 $425 Buy Now
MR synthRE Mineralocorticoid Receptor Get Info 5 µg 32155 $425 Buy Now
MTF1 synthRE Heavy Metal Stress Get Info 5 µg 32156 $425 Buy Now
c-Myc synthRE c-Myc Get Info 5 µg 32157 $425 Buy Now
Nanog synthRE Stem Cell Pluripotency Get Info 5 µg 32158 $425 Buy Now
NFAT synthRE PKC/Ca++ Get Info 5 µg 32159 $425 Buy Now
NFκB synthRE Inflammation Get Info 5 µg 32160 $425 Buy Now
Nrf1 synthRE Nuclear Respiratory Factor Get Info 5 µg 32161 $425 Buy Now
NRSF synthRE Neuron Silencing Factor Get Info 5 µg 32162 $425 Buy Now
Oct-4 synthRE Stem Cell Pluripotency Get Info 5 µg 32163 $425 Buy Now
p53 synthRE v1 DNA Damage, Apoptosis Get Info 5 µg 32164 $425 Buy Now
p53 synthRE v2 DNA Damage, Apoptosis Get Info 5 µg 32165 $425 Buy Now
PAX6 synthRE PAX6 Get Info 5 µg 32166 $425 Buy Now
PPAR synthRE Peroxisome Proliferator-Activated Receptor Get Info 5 µg 32167 $425 Buy Now
PPAR gamma synthRE Peroxisome Proliferator-Activated Receptor Gamma Get Info 5 µg 32168 $425 Buy Now
PR synthRE Progesterone Receptor Get Info 5 µg 32169 $425 Buy Now
RARE synthRE Retinoic Acid Get Info 5 µg 32170 $425 Buy Now
RBP-JK synthRE Notch Get Info 5 µg 32171 $425 Buy Now
c-Rel synthRE NFkB/Inflammation Get Info 5 µg 32118 $425 Buy Now
ROR alpha 1 synthRE Retinoid-Related Orphan Receptor Alpha2 Get Info 5 µg 32172 $425 Buy Now
ROR alpha 2 synthRE Retinoid-Related Orphan Receptor Alpha2 Get Info 5 µg 32173 $425 Buy Now
ROR beta synthRE Retinoid-Related Orphan Receptor Beta Get Info 5 µg 32174 $425 Buy Now
RORE synthRE Retinoid-Related Orphan Receptor Get Info 5 µg 32175 $425 Buy Now
RXR synthRE Retinoid X Receptor Get Info 5 µg 32176 $425 Buy Now
Sox2 synthRE Stem Cell Pluripotency Get Info 5 µg 32179 $425 Buy Now
Sp1 synthRE v1 Specificity Protein 1 Get Info 5 µg 32180 $425 Buy Now
Sp1 synthRE v2 Specificity Protein 1 Get Info 5 µg 32181 $425 Buy Now
SRF synthRE Serum Response Get Info 5 µg 32182 $425 Buy Now
STAT1 synthRE Inflammation Get Info 5 µg 32183 $425 Buy Now
STAT3 synthRE Inflammation Get Info 5 µg 32184 $425 Buy Now
STAT4 synthRE Inflammation Get Info 5 µg 32185 $425 Buy Now
TCF-LEF synthRE Wnt Get Info 5 µg 32186 $425 Buy Now
TGF Beta synthRE v1 TGF Beta Get Info 5 µg 32177 $425 Buy Now
TGF Beta synthRE v2 TGF Beta Get Info 5 µg 32178 $425 Buy Now
TR synthRE v1 Thyroid Receptor Get Info 5 µg 32187 $425 Buy Now
TR synthRE v2 Thyroid Receptor Get Info 5 µg 32188 $425 Buy Now
VDR synthRE v1 Vitamin D Receptor Get Info 5 µg 32189 $425 Buy Now
VDR synthRE v2 Vitamin D Receptor Get Info 5 µg 32190 $425 Buy Now
YY1 synthRE Yin Yang 1 Get Info 5 µg 32191 $425 Buy Now

Each Synthetic Response Element reporter construct includes an optimized synthetic response element that is made up of multiple repeats of a transcription factor binding site motif. In contrast to our LightSwitch Promoter Reporter vectors, which contain endogenous human promoters, Synthetic Response Element vectors contain the synthetic element cloned upstream of a minimal HSV-TK promoter and the RenSP luciferase reporter gene in the pLightSwitch_LR vector. This makes the empty pLightSwitch_LR vector ideal for use as a negative control, as it contains only a basal promoter. For complete information on the pLightSwitch_LR vector, please see the Empty LightSwitch™ Reporter Vectors page.

pLightSwitch_LR vector diagram

Applications of Synthetic Response Elements:

  • Use as a primary screen to identify compounds or conditions that affect the activity of a biological pathway. Follow up using related endogenous promoter reporter constructs to detect subtle differences between compounds or conditions.
  • Generate dose response curves to various compounds or conditions.
  • Use as a positive control in your pathway-specific, cell-based assays.

AR Synthetic Response Element (Androgen Receptor)

HT1080 fibrosarcoma cells were co-transfected with the AR synthRE reporter construct (Cat. No. 32107) and an AR cDNA construct according to our standard protocols. Half of the transfected cells were treated with 10 nM R1881 for 24 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch AR synthRE reporter construct

CREB Synthetic Response Element

HT1080 fibrosarcoma cells were transfected with the CREB synthRE reporter construct (Cat. No. 32117) according to our standard protocols. Half of the transfected cells were treated with 100 nM PMA for 4 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch CREB synthRE reporter construct

ER Synthetic Response Element (Estrogen Receptor)

HT1080 fibrosarcoma cells were co-transfected with the ER synthRE reporter construct (Cat. No. 32125) and an ER cDNA construct according to our standard protocols. Half of the transfected cells were treated with 10 nM β-estradiol for 24 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch ER synthRE reporter construct

GR Synthetic Response Element (Glucocorticoid Receptor)

HT1080 fibrosarcoma cells were transfected with the GR synthRE reporter construct (Cat. No. 32140) according to our standard protocols. Half of the transfected cells were treated with 100 nM dexamethasone (DMSO) for 4 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch GR synthRE reporter construct

HIF-1 alpha Synthetic Response Element (Hypoxia)

HT1080 fibrosarcoma cells were transfected with the HIF-1 alpha synthRE reporter construct (Cat. No. 32141) according to our standard protocols. Half of the transfected cells were treated with a low oxygen condition (1% O2) for 24 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch HIF-1 alpha synthRE reporter construct

HSF Synthetic Response Element (Heat Shock)

HT1080 fibrosarcoma cells were transfected with the HSF synthRE reporter construct (Cat. No. 32144) according to our standard protocols. The transfected cells were then incubated at 37°C or 43°C for 8 hours. The cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch HSF synthRE reporter construct

NFκB Synthetic Response Element (Inflammation)

HT1080 fibrosarcoma cells were transfected with the NFκB synthRE reporter construct (Cat. No. 32160) according to our standard protocols. Half of the transfected cells were treated with 50 ng/ml TNF-α for 8 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch NFkB synthRE reporter construct

STAT1 Synthetic Response Element (Inflammation)

HT1080 fibrosarcoma cells were transfected with the STAT1 synthRE reporter construct (Cat. No. 32183) according to our standard protocols. Half of the transfected cells were treated with 500 U/ml Interferon-α for 8 hours. The untreated and treated cells were then assayed using the LightSwitch Luciferase Assay Kit.

Diagram depicting the luciferase activity of the LightSwitch STAT1 synthRE reporter construct