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Recombinant Proteins & Enzymes

Recombinant SMARCA2 / BRM (1367-1511), GST-Tag

Aliases: SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a
Catalog No: 31481 Format: 100 µg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SMARCA2 / BRM (1367-1511), GST-TagContents

100 µg of recombinant SMARCA2 / BRM (1367-1511), GST-Tag protein expressed in E. coli and supplied in 25 mM Tris pH 7.4, 150 mM NaCl and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SMARCA2 / BRM (1367-1511), GST-TagBackground

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2), also known as BRM, is a member of the SWI/SNF family of proteins and is similar to the Brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA2 contains bromodomains for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR). SMARCA1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). SMARCA2 plays a pivotal role in the regulation of the switch in subunit composition of the npBAF and nBAF complexes as cells transition from proliferating neural stem/progenitor cells to post-mitotic neurons during neural development.

for Recombinant SMARCA2 / BRM (1367-1511), GST-TagApplication Notes

Recombinant SMARCA2 / BRM (1367-1511), GST-Tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant SMARCA2 / BRM (1367-1511), GST-TagProtein Details

The peptide corresponding to amino acids 1367-1511 that contains the bromodomain sequences of SMARCA2 / BRM (accession number NP_003061.3) was expressed in E. coli and contains an N-terminal GST-Tag with an observed molecular weight of 44.2 kDa. It shows binding specificity for acetylated H3K9, H3K14, H3K9/14, H4K8, H4K12, H4K16 and H4K5/8/12/16. The recombinant protein is >95% pure by SDS-PAGE.

 
SMARCA2 / BRM (1367-1511), GST-Tag Coomassie gel

Recombinant SMARCA2 / BRM (1367-1511), GST-Tag protein gel.
SMARCA2 / BRM (1367-1511), GST-Tag protein was run on an SDS-PAGE gel and stained with Coomassie blue.

SMARCA2 / BRM (1367-1511) HTRF activity assay

Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay
3.3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.

SMARCA2 / BRM (1367-1511) HTRF activity assay

Recombinant SMARCA2 / BRM (1367-1511) HTRF activity assay
3.3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA2 / BRM (1367-1511) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.

for Recombinant SMARCA2 / BRM (1367-1511), GST-TagStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SMARCA2 / BRM (1367-1511), GST-TagGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant SMARCA2 / BRM (1367-1511), GST-Tag
 

Data Thumbnails

SMARCA2 / BRM (1367-1511), GST-Tag Coomassie gel

SMARCA2 / BRM (1367-1511), GST-Tag Coomassie stained gel.
(Click image to enlarge and see details).

SMARCA2 / BRM (1367-1511) HTRF activity assay

SMARCA2 / BRM (1367-1511) HTRF activity assay.
(Click image to enlarge and see details).

SMARCA2 / BRM (1367-1511) HTRF activity assay

SMARCA2 / BRM (1367-1511) HTRF activity assay.
(Click image to enlarge and see details).