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Recombinant Proteins & Enzymes

Recombinant AGO2 protein

Aliases: Q10; EIF2C2
Catalog No: 31486 Format: 20 µg $395 Buy Now
Catalog No: 31886 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant AGO2 proteinContents

Full length recombinant AGO2 protein expressed in Sf9 cells and is provided in 25 mM HEPES, pH 7.5, 500 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP and 0.2 mg/ml 3X FLAG peptide. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant AGO2 proteinBackground

AGO2 (Argonaute RISC Catalytic Component 2, also known as Q10; EIF2C2) is a member of the Argonaute family of proteins which play a role in RNA interference. This process is important both in gene regulation by microRNAs and in defense against viral infections. The RNA-induced silencing complex, or RISC, is a multiprotein complex that includes argonaute proteins and one strand of a small interfering RNA (siRNA) or microRNA (miRNA). RISC uses the siRNA or miRNA as a template for recognizing complementary mRNA. When it recognizes a complementary strand, it activates AGO2 to cleave the mRNA. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. In some cases, RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3’ untranslated region (3’UTR). In addition, AGO2 can also up-regulate the posttranscriptional upregulation of specific mRNAs under certain growth conditions.

for Recombinant AGO2 proteinApplication Notes

Recombinant AGO2 is suitable for use in the study of miRNA screening, RNAi research and selectivity profiling.

Specific Activity: RNA endonuclease activity.

for Recombinant AGO2 proteinProtein Details

Recombinant AGO2 (accession number NP_036286.2) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 104 kDa. The recombinant protein is >90% pure by SDS-PAGE.

 
AGO2 Coomassie gel

Recombinant AGO2 protein gel.
AGO2 protein was run on an SDS-PAGE gel and stained with Coomassie blue.

for Recombinant AGO2 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant AGO2 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant AGO2 protein
 

Data Thumbnails

AGO2 Coomassie gel

AGO2 Coomassie stained gel.
(Click image to enlarge and see details).