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Antibodies

Ago1/2/3 antibody (mAb)

Clone: 2A8
Catalog No: 39937 Format: 100 µg $340 Buy Now
Catalog No: 39938 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG
Purification:Protein G Chromatography
Applications:ChIP, IF, IHC, IP, WB
Reactivity:Human
Molecular Weight:95 kDa

Applications Table

Western Blot Chromatin IP IF IP
0.5 - 2 µg/ml 10 µg per ChIP 1 - 2 µg/ml 10 µg per IP

for Ago1/2/3 antibody (mAb) (Clone 2A8)Background

The Argonaute family of proteins (Ago1, Ago2, Ago3 and Ago4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Ago3 lacks RNase activity and does not appear to cleave target mRNA molecules. Argonaute family proteins contain a PAZ and a PIWI domain.

for Ago1/2/3 antibody (mAb) (Clone 2A8)Immunogen

This Ago1/2/3 antibody was raised against a recombinant protein corresponding to amino acids 47-879 of human Ago2.

for Ago1/2/3 antibody (mAb) (Clone 2A8)Application Notes

This Argonaute antibody has been validated for use in chromatin immunoprecipitation (ChIP, 10 µg per ChIP), immunofluorescence (1 - 2 µg/ml), immunohistochemistry, immunoprecipitation (10 µg per IP) and Western blotting (0.5 - 2 µg/ml). It recognizes Ago1, Ago2 and Ago3 proteins.

It has also been validated for use in HITS-CLIP (high-throughput sequencing of RNA isolated by crosslinking immunoprecipitation) and RIP-ChIP. See References for additional information.

for Ago1/2/3 antibody (mAb) (Clone 2A8)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Chromatin IP Assays

Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Ago1/2/3 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Ago1/2/3 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Ago1/2/3 antibody (mAb).

for Ago1/2/3 antibody (mAb) (Clone 2A8)References

This antibody has been used in the following publications:
ChIP: Schwartz JC et al (2009) Nat Struct Molec Biol 15: 842-848.
IF: Nelson PT et al (2007) RNA 13: 1787-1792.
IHC: Nelson PT et al (2007) RNA 13: 1787-1792.
IP: Nelson PT et al (2007) RNA 13: 1787-1792.
HITS-CLIP: Chi SW et al (2009) Nature 460: 479-486.
RIP-ChIP: Schwartz JC et al (2009) Nat Struct Molec Biol 15: 842-848.
WB: Nelson PT et al (2007) RNA 13: 1787-1792.

for Ago1/2/3 antibody (mAb) (Clone 2A8)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Ago1/2/3 antibody (mAb) (Clone 2A8)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting