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Antibodies

NFκB p50 antibody (pAb)

Aliases: p50, KBF1, p105, EBP-1, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B
Catalog No: 39037 Format: 200 µl $410 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:50 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for NFkB p50 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Published Applications: see references

For optimal results, primary antibody incubations should be performed at 4°C. Individual optimization may be required.

for NFkB p50 antibody (pAb)Immunogen

This NFkB p50 antibody was raised against a synthetic peptide corresponding to amino acid residues 363-376 of human NFκB p50.

for NFkB p50 antibody (pAb)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for NFkB p50 antibody (pAb)References

 
NFκB p50 antibody (pAb) tested by Western blot.

NFκB p50 antibody tested by Western blot.
Detection of NFκB p50 by Western blot analysis. NFκB p50 can be detected in nuclear extracts derived from Raji cells using NFκB p50 rabbit polyclonal antibody. Lanes 2-4 demonstrate progressive titration of the antibody at 1:100, 1:500 and 1:1,000 dilutions, while lane 1 illustrates elimination of the signal in the presence of the immunizing peptide.

for NFkB p50 antibody (pAb)Background

NFκB p50 is a member of the NFκB family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFκB p65, NFκB3) and RelB (I-Rel). Rel proteins form dimers with the NFκB p50 (proteolytically processed from p105/NFκB1) and p52 (proteolytically processed from p100/NFκB2) members of the NFκB family. The NFκB p65/p50 heterodimer is the most abundant form of NFκB.

NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB. The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ). Upon its activation, the IKK complex phosphorylates IκB proteins (IκBα, IκBα phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFκB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

for NFkB p50 antibody (pAb)Positive Control

Active Motif's Raji nuclear extract (Catalog No. 36023) can be used as a positive control for NFκB p50 antibody.

for NFkB p50 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NFkB p50 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

NFκB p50 antibody (pAb)
 

Data Thumbnails

NFκB p50 antibody (pAb) tested by Western blot.

Western blot of NFκB p50 pAb.
(Click image to enlarge and see details.)