Histone H3 dimethyl Lys79 antibody (pAb)

for Histone H3 dimethyl Lys79 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine 79 of histone H3 can be mono-, di- or trimethylated by Dot1 methylase; methylation at this residue acts as a marker of inactive chromatin regions that is critical for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.

for Histone H3 dimethyl Lys79 antibody (pAb)Immunogen

This antibody was raised against a peptide including dimethyl-lysine 79 of histone H3.

for Histone H3 dimethyl Lys79 antibody (pAb)Application Notes

This Histone H3 dimethyl Lys79 antibody (H3K79me2) has been validated for use in chromatin IP (10 µl per ChIP), ChIP-Seq & ChIP-chip (5 µl each) and Western blotting (1:500 - 1:5,000). Specificity for dimethyl lysine 79 was confirmed by dot blot with non-modified histone H3 peptide or peptides mono-, di- or trimethylated at lysine 79. In addition, Western blot against 200 ng of recombinant histone H3 was negative. This product is specific for histone H3 dimethylated at lysine 79. Because this modification is conserved from yeast to human, a wide range of reactivity is expected.

The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H3 dimethyl Lys79 antibody was validated for ChIP-Seq in the study (see References).

for Histone H3 dimethyl Lys79 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide.

for Histone H3 dimethyl Lys79 antibody (pAb)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 dimethyl Lys79 antibody.

for Histone H3 dimethyl Lys79 antibody (pAb)References

This antibody has been used as described below:

for Histone H3 dimethyl Lys79 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H3 dimethyl Lys79 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.