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Mi-2 beta antibody (pAb)
| Catalog No: 39289 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39290 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:500 - 1:1,000 | — | 1:500 - 1:2,000 |
for Mi-2 beta antibody (pAb)Background
Mi-2β (Mi-2beta, CHD4, chromodomain helicase DNA-binding protein 4) represents the major Mi-2 isoform present in the complex Mi-2/NuRD. This complex is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. In addition to the ATPase domain, Mi-2 beta contains a chromodomain and two PHD domains. Mi-2 beta interacts with BRG-1, a component of the SWI/SNF complex, and the RET finger protein (RFP).
for Mi-2 beta antibody (pAb)Immunogen
This Mi-2B antibody was raised against a recombinant protein corresponding to amino acids 22-155 of mouse Mi-2 beta.
for Mi-2 beta antibody (pAb)Application Notes
This Mi-2B antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution) and immunofluorescence (1:500 - 1:2,000 dilution).
for Mi-2 beta antibody (pAb)Buffer
Rabbit serum containing 30% glycerol, 0.035% sodium azide.
for Mi-2 beta antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Mi-2 beta antibody.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Mi-2 beta antibody (pAb).
for Mi-2 beta antibody (pAb)References
Ramirez-Carrozzi et al (2006) Genes Dev. 20:282-296.
Mi-2 beta antibody tested by Western blot.
Whole-cell extract of mouse splenocytes stained with Mi-2 beta antibody (1:1,1000).
Mi-2 beta antibody tested by immunofluorescence.
Top left: HeLa cells stained with Mi-2 beta antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.
for Mi-2 beta antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Mi-2 beta antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
