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Antibodies

Mi-2 beta antibody (pAb)

Aliases: CHD4, Mi-2b
Catalog No: 39289 Format: 100 µl $410 Buy Now
Catalog No: 39290 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:240 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

for Mi-2 beta antibody (pAb)Application Notes


Validated Applications:
ICC/IF: 1:500 - 1:2,000 dilution
WB: 1:500 - 1:1,000 dilution

Published Applications:
ICC/IF
IHC
See references for more information. Individual optimization may be required.

for Mi-2 beta antibody (pAb)Immunogen

This Mi-2B antibody was raised against a recombinant protein corresponding to amino acids 22-155 of mouse Mi-2 beta.

for Mi-2 beta antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Mi-2 beta antibody (pAb)References

 
Mi-2 beta antibody (pAb) tested by immunofluorescence.

Mi-2 beta antibody tested by immunofluorescence.
Top left: HeLa cells stained with Mi-2 beta antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.

Mi-2 beta antibody (pAb) tested by Western blot.

Mi-2 beta antibody tested by Western blot.
Whole-cell extract of mouse splenocytes stained with Mi-2 beta antibody (1:1,1000).

for Mi-2 beta antibody (pAb)Background

Mi-2β (Mi-2beta, CHD4, chromodomain helicase DNA-binding protein 4) represents the major Mi-2 isoform present in the complex Mi-2/NuRD. This complex is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. In addition to the ATPase domain, Mi-2 beta contains a chromodomain and two PHD domains. Mi-2 beta interacts with BRG-1, a component of the SWI/SNF complex, and the RET finger protein (RFP).

for Mi-2 beta antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Mi-2 beta antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Mi-2 beta antibody (pAb).

for Mi-2 beta antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Mi-2 beta antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Mi-2 beta antibody (pAb)
 

Data Thumbnails

Mi-2 beta antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Mi-2 beta pAb.
(Click image to enlarge and see details.)

Mi-2 beta antibody (pAb) tested by Western blot.

Western blot of Mi-2 beta pAb.
(Click image to enlarge and see details.)