YOUR CART
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| Western Blot | Chromatin IP |
|---|---|
| 1:5,000 - 1:50,000 | 10 µl per ChIP |
| View Technical Data Sheet (TDS) |
Background for PARP-1 N-terminal antibody (pAb) antibody (pAb):
PARP-1 N-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis.For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section or our website.
Chromatin IP Assays:
Active Motif has Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page
Immunogen for PARP-1 N-terminal antibody (pAb) antibody (pAb):
His-tagged fusion protein corresponding to the N-terminal half of human PARP-1 was used to generate this PARP-1 antibody.Application Notes for PARP-1 N-terminal antibody (pAb) antibody (pAb):
This PARP-1 N-terminal antibody has been validated for use in Western blotting (1:5,000 - 1:50,000 dilution).Buffer:
Rabbit serum containing 30% glycerol, 0.035% sodium azide.Positive Control:
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for PARP-1 N-terminal antibody.Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
References:
This antibody has been used in the following references:Kim MY et al (2004) Cell 119: 803-814.
Krishnakumar R et al (2008) Science 319: 819-821.
PARP-1 N-terminal antibody tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with PARP-1 N-terminal antibody at a dilution of 1:20,000.
PARP-1 N-terminal antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT™ Express Kit (Catalog No. 53008) and MCF7 chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µl of PARP-1 N-terminal antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the BRCA2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Storage:
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.Guarantee:
This product is guaranteed for 6 months from date of receipt.This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
