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Antibodies

CUL4B antibody (pAb)

Aliases: SFM2, MRXSC, MRXHF2, MRXS15
Catalog No: 61445 Format: 100 µl $410 Buy Now
Catalog No: 61446 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:110 kDa
Reactivity:Human

Applications

Western Blot Validated

for CUL4B antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for CUL4B antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human CUL4B.

for CUL4B antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CUL4B antibody (pAb)References

 
CUL4B antibody (pAb) tested by Western blot.

CUL4B (pAb) tested by Western blot.
Detection of CUL4B by Western blot analysis. Nuclear extract of MCF-7 cells (20 µg) was probed with CUL4B (pAb) at a 1:500 dilution.

for CUL4B antibody (pAb)Background

CUL4B (Cullin 4B) is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, Histone H2A, Histone H3 and Histone H4 in response to radiation-induced DNA damage and may act as a transcriptional corepressor by ubiquitination of Histone H2AK119 and recruiting PRC2 complexes.

for CUL4B antibody (pAb)Positive Control

Active Motif's MCF-7 nuclear extract (Catalog No. 36017) can be used as a positive control for CUL4B antibody.

for CUL4B antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CUL4B antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CUL4B antibody (pAb)
 

Data Thumbnails

CUL4B antibody (pAb) tested by Western blot.

Western blot of CUL4B pAb.
(Click image to enlarge and see details.)