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Antibodies

Histone H3.1S28ph antibody (mAb)

Clone: 5D10D4
Catalog No: 61697 Format: 100 µg $410 Buy Now
Catalog No: 61698 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for Histone H3.1S28ph antibody (mAb) (Clone 5D10D4)Application Notes


Validated Applications:
ICC/IF: 2 - 10 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

for Histone H3.1S28ph antibody (mAb) (Clone 5D10D4)Immunogen

This antibody was raised against a peptide corresponding to phospho-serine 28 of human Histone H3.1. This antibody does not react with Histone H3.3 phosphorylated on serine 28.

for Histone H3.1S28ph antibody (mAb) (Clone 5D10D4)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H3.1S28ph antibody (mAb) (Clone 5D10D4)References

 
Histone H3.1S28ph antibody (mAb) tested by immunofluorescence.

Histone H3.1S28ph antibody (mAb) (Clone 5D10D4) tested by Immunofluorescence.
Left: Formaldehyde fixed mitotic DM4 cell stained with Histone H3.1S28ph antibody (mAb).
Right: Hoechst stain.

Histone H3.1S28ph antibody (mAb) tested by Western blot.

Histone H3.1S28ph antibody (mAb) (Clone 5D10D4) tested by Western blot.
Detection of Histone H3.1S28ph antibody by Western blot. The analysis was performed using 20 µg of untreated (lane 1) or colcemid treated (lane 2) HeLa nuclear extract with Histone H3.1S28ph antibody at a 1 µg/ml dilution.

for Histone H3.1S28ph antibody (mAb) (Clone 5D10D4)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. There are three protein variants of Histone H3, Histone H3.1, 3.2 and 3.3. The incorporation of Histone H3.1 and H3.2 into nucleosomes is replication dependent, in contrast to Histone H3.3, which is independent of DNA synthesis and occurs throughout the cell cycle. Human Histone H3.1 and H3.3 are identical in amino acid sequences except at position 110 where H3.1 has a cysteine and H3.3 has a serine. Phosphorylation of serine 28 occurs in early mitosis when chromosomes begin to condense and during premature chromosome condensation induced in S-phase cells.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3.1S28ph antibody (mAb).

for Histone H3.1S28ph antibody (mAb) (Clone 5D10D4)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3.1S28ph antibody (mAb) (Clone 5D10D4)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3.1S28ph antibody (mAb)
 

Data Thumbnails

Histone H3.1S28ph antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H3.1S28ph mAb.
(Click image to enlarge and see details.)

Histone H3.1S28ph antibody (mAb) tested by Western blot.

Western blot of Histone H3.1S28ph mAb.
(Click image to enlarge and see details.)