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Antibodies

PKC-B antibody (pAb)

Aliases: PRKCB, PKCB, PRKCB1, PRKCB2, PKC-beta
Catalog No: 61733 Format: 100 µl $410 Buy Now
Catalog No: 61734 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:85 kDa
Reactivity:Human

Applications

Western Blot Validated

for PKC-B antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

for PKC-B antibody (pAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human PKC-B.

for PKC-B antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
PKC-B antibody (pAb) tested by Western blot.

PKC-B antibody (pAb) tested by Western blot.
PKC-B detection by Western blot. The analysis was performed using 20 µg of K562 nuclear cell extract and PKC-B antibody at a 1:500 dilution.

for PKC-B antibody (pAb)Background

PKC-B (Protein Kinase C, Beta) ia a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at Ser-559, Ser-644 and Ser-652. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at Ser-180, which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage.

Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of Thr-6 of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 Lys-4 (H3K4me) by LSD1/KDM1A. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.

for PKC-B antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PKC-B antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PKC-B antibody (pAb)
 

Data Thumbnails

PKC-B antibody (pAb) tested by Western blot.

Western blot of PKC-B pAb.
(Click image to enlarge and see details.)