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Recombinant Proteins & Enzymes

Recombinant PRMT4 (CARM1) protein

Catalog No: 81107 Format: 10 µg $405 Buy Now
Catalog No: 81807 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant PRMT4 (CARM1) proteinContents

Full length recombinant PRMT4 (CARM1) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant PRMT4 (CARM1) proteinBackground

PRMT4 (CARM1) (Coactivator-associated arginine methyltransferase 1 or Carm1) is a protein arginine N-methyltransferase that catalyzes the mono- and asymmetric dimethylation of arginine residues in its substrate proteins. It serves as a coactivator of transcription and also plays a role in nuclear receptors. PRMT4 (CARM1) methylates histone H3 at Arg2, Arg17 and Arg26; it also methylates a number of non-histone substrates involved in the regulation and mechanism of gene expression.

for Recombinant PRMT4 (CARM1) proteinApplication Notes

Recombinant PRMT4 (CARM1) protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant PRMT4 (CARM1) proteinProtein Details

Recombinant human PRMT4 (CARM1) was expressed in a baculovirus expression system as the full length protein (accession number NP_954592.1) with an N-terminal Flag tag. The molecular weight of the protein is 66.8 kDa.

 
Recombinant PRMT4 (CARM1) protein gel

Recombinant PRMT4 (CARM1) protein gel
8% SDS-PAGE Coomassie staining
MW: 66.8 kDa
Purity: >88%

ecombinant PRMT4 (CARM1) protein activity assay

Recombinant PRMT4 (CARM1) protein activity assay
0.5 µg Histone H3.1 (Cat. No. 31294) were incubated with 0, 0.05 µg, 0.1 µg, 0.2 µg PRMT4 (CARM1) in 30 µl reaction system for 2 hours at room temperature, respectively. 6 µl reaction samples were loaded and run on a 13% SDS-PAGE gel. Western blot was used to detect the generation of products (H3R17me2a antibody, Cat. No. 39709, 1:1000 dilution).  The western blot results show that histone H3 is di-methylated on its Arginine 17 by PRMT4 (CARM1).  

for Recombinant PRMT4 (CARM1) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant PRMT4 (CARM1) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant PRMT4 (CARM1) protein
 

Data Thumbnails

Recombinant PRMT4 (CARM1) protein gel

Recombinant PRMT4 (CARM1) protein gel
(Click to enlarge and view details)

ecombinant PRMT4 (CARM1) protein activity assay

Recombinant PRMT4 (CARM1) protein activity assay
(Click to enlarge and view details)