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Recombinant Proteins & Enzymes

Recombinant MLLT3 / AF9 (1-138) protein

Catalog No: 81124 Format: 50 µg $395 Buy Now
Catalog No: 81824 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant MLLT3 / AF9 (1-138) proteinContents

Recombinant MLLT3 / AF9 (1-138) protein is supplied in 25 mM Tris-HCl pH 7.4, 500 mM NaCl, 2 mM EDTA, 2 mM DTT, 10% glycerol, 0.5 mM TCEP.

for Recombinant MLLT3 / AF9 (1-138) proteinBackground

MLLT3 (Myeloid/Lymphoid Or Mixed-Lineage Leukemia Translocated To Chromosome 3 Protein), also known as YEATS3 or AF9, is a chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. It specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. MLLT3 recognizes and binds histone H3 crotonylated at Lys-9 (H3K9cr), and with slightly lower affinity histone H3 crotonylated at Lys-18 (H3K18cr). It also recognizes and binds histone H3 acetylated at Lys-9 (H3K9ac), but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones.

for Recombinant MLLT3 / AF9 (1-138) proteinApplication Notes

This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Binding Assay for Recombinant MLLT3 / AF9 (1-138) protein: 0.25 nmol biotinylated H3K9ac peptide was incubated with 0.25 nmol MLLT3 / AF9 (1-138) protein in binding buffer containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100. Peptide-protein compounds were incubated with streptavidin beads for 4 hours at 4°C, and pull-down products were run on a 10% SDS-PAGE gel, stained with Coomassie Blue. (Single MLLT3 / AF9 (1-138) protein was used as negative control). * indicates streptavidin.

for Recombinant MLLT3 / AF9 (1-138) proteinProtein Details

Recombinant MLLT3 / AF9 (1-138) protein was expressed in E.coli as the full length protein (accession number NP_004520.2) with an N-terminal 6xHis Tag. The molecular weight of the protein is 19.8 kDa.

 
Recombinant MLLT3 / AF9 (1-138) protein gel

Recombinant MLLT3 / AF9 (1-138) protein gel
13% SDS-PAGE Coomassie staining
MW: 19.8 kDa
Purity: >60%

Binding Assay for Recombinant MLLT3 / AF9(1-138) protein

Binding Assay for Recombinant MLLT3 / AF9 protein
0.25 nmol biotinylated H3K9ac peptide was incubated with 0.25 nmol MLLT3 / AF9 (1-138) protein in binding buffer. Peptide-protein compounds were incubated with streptavidin beads and pull-down products were run on SDS-PAGE gel, stained with Coomassie Blue. (Single MLLT3 / AF9 protein used as negative control). * indicates Streptavidin.

for Recombinant MLLT3 / AF9 (1-138) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant MLLT3 / AF9 (1-138) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant MLLT3 / AF9 (1-138) protein
 

Data Thumbnails

Recombinant MLLT3 / AF9 (1-138) protein gel

Recombinant MLLT3 / AF9 (1-138) protein gel
(Click to enlarge and view details)

Binding Assay for Recombinant MLLT3 / AF9(1-138) protein

Binding Assay for Recombinant MLLT3 / AF9 (1-138) protein
(Click to enlarge and view details)