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Histone H3 acetyl Lys14 antibody (pAb)
| Catalog No: 39599 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39616 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | 10 µl per ChIP |
for Histone H3 acetyl Lys14 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.
for Histone H3 acetyl Lys14 antibody (pAb)Immunogen
This Histone H3 acetyl Lys14 antibody was raised against a peptide including acetyl-lysine 14 of histone H3.
for Histone H3 acetyl Lys14 antibody (pAb)Application Notes
This Histone H3 acetyl Lys14 antibody (H3K14Ac) has been validated for use in chromatin IP (10 µl per ChIP) and in Western blotting (1:500 - 1:2,000 dilution). It is not recommended for immunofluorescence.
This antibody was immunodepleted to remove cross-reactivity toward other sites of H3 acetylation.
for Histone H3 acetyl Lys14 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H3 acetyl Lys14 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys14 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 acetyl Lys14 antibody (pAb).
Histone H3 acetyl Lys14 antibody tested by Western blot.
HeLa acid extract (10 µg per lane) was probed with Histone H3 acetyl Lys14 antibody (1:1,000 dilution).
Lane 1: No treatment.
Lane 2: Cells treated with sodium butyrate.
Histone H3 acetyl Lys14 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys14 pAb for acetyl Lys14 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: Unmodified histone H3 Lys14 peptide.
Lane 2: Acetyl-Lys14 peptide.
Lane 3: Acetyl-Lys9 peptide.
Lane 4: Acetyl-Lys18 peptide.
Lane 5: Acetyl-Lys23 peptide.
Lane 6: Acetyl-Lys27 peptide.
Lane 7: Acetyl-Lys37 peptide.
Lane 8: Acetyl-Lys36 peptide.
Lane 9: Histone H2A acetyl-Lys5 peptide.
Lane 10: Histone H2A acetyl-Lys9 peptide.
Histone H3 acetyl Lys14 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µl of Histone H3 acetyl Lys14 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
for Histone H3 acetyl Lys14 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 acetyl Lys14 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
