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Histone H3 dimethyl Lys36 antibody (pAb)
| Catalog No: 39891 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39892 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1 - 2 µg/ml | Not tested. ChIP-validated version available: 39255. |
for Histone H3 dimethyl Lys36 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.
for Histone H3 dimethyl Lys36 antibody (pAb)Immunogen
This Histone H3 dimethyl Lys36 antibody was raised against a peptide including dimethyl-lysine 36 of histone H3.
for Histone H3 dimethyl Lys36 antibody (pAb)Application Notes
This Histone H3 dimethyl Lys36 antibody (H3K36me2) has been validated for use in Chromatin immunoprecipitation (3 - 5 µl per ChIP), Western blotting (1:2,500 - 1:5,000 dilution) and immunofluorescence (1:250 - 1:1,000 dilution).
Detection of histone H3 with this antibody disappears completely in Western blots of a yeast strain deleted for the Set2 gene, which encodes for the Lys36 methyltransferase.
for Histone H3 dimethyl Lys36 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H3 dimethyl Lys36 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 dimethyl Lys36 antibody.
Histone H3 dimethyl Lys36 antibody tested by Western blot.
Nuclear extract of HeLa cells (20 µg) probed with Histone H3 dimethyl Lys36 antibody at a dilution of 1 µg/ml.
Histone H3 dimethyl Lys36 antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys36 antibody for dimethyl Lys36 histone H3. Methylated peptides corresponding to the immunogen and related sequences derived from histone H3 were spotted onto PVDF and probed with Histone H3 dimethyl Lys36 antibody at 1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Top panel:
Lane 1: unmodified lysine 4 peptide.
Lane 2: monomethyl lysine 4 peptide.
Lane 3: dimethyl lysine 4 peptide.
Lane 4: trimethyl lysine 4 peptide.
Lane 5: unmodified lysine 9, 14, 18 peptide.
Lane 6: monomethyl lysine 9 peptide.
Lane 7: dimethyl lysine 9 peptide.
Lane 8: trimethyl lysine 9 peptide.
Lane 9: dimethyl lysine 14 peptide.
Lane 10: monomethyl lysine 18 peptide.
Lane 11: dimethyl lysine 18 peptide.
Lane 12: trimethyl lysine 18 peptide.
Bottom panel:
Lane 1: Unmodified Lys23 peptide.
Lane 2: Monomethyl Lys23 peptide.
Lane 3: Dimethyl Lys23 peptide.
Lane 4: Trimethyl Lys23 peptide.
Lane 5: unmodified Lys27 peptide.
Lane 6: monomethyl Lys27 peptide.
Lane 7: dimethyl Lys27 peptide.
Lane 8: trimethyl Lys27 peptide.
Lane 9: unmodified Lys36 peptide.
Lane 10: monomethyl Lys36 peptide.
Lane 11: dimethyl Lys36 peptide.
Lane 12: trimethyl Lys36 peptide.
for Histone H3 dimethyl Lys36 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 dimethyl Lys36 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
