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Histone H3 monomethyl Lys79 antibody (pAb)
| Catalog No: 39921 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39922 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1 - 2 µg/ml | 10 µg per ChIP |
for Histone H3 monomethyl Lys79 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Lysine 79 of histone H3 can be mono-, di- or trimethylated by Dot1 methylase; methylation at this residue acts as a marker of inactive chromatin regions that is critical for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.
for Histone H3 monomethyl Lys79 antibody (pAb)Immunogen
This Histone H3 monomethyl Lys79 antibody was raised against a peptide including monomethyl-lysine 79 of histone H3.
for Histone H3 monomethyl Lys79 antibody (pAb)Application Notes
This Histone H3 monomethyl Lys79 antibody has been validated for use in chromatin immunoprecipitation (ChIP, 10 µg per ChIP) and Western blotting (1 - 2 µg/ml).
Addition of 0.05% Tween-20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
for Histone H3 monomethyl Lys79 antibody (pAb)Buffer
PBS pH 7.5, 30% glycerol, 0.035% sodium azide
for Histone H3 monomethyl Lys79 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 monomethyl Lys79 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 monomethyl Lys79 antibody (pAb).
Histone H3 monomethyl Lys79 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (20 µg) probed with Histone H3 monomethyl Lys79 antibody (1 µg/ml dilution).
Histone H3 monomethyl Lys79 antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys79 antibody for monomethyl-Lys79 of histone H3. Peptides corresponding to the region around lysine 79 of histone H3 were spotted onto PVDF and probed with Histone H3 monomethyl Lys79 antibody at a dilution of 1 µg/ml. The amount of peptide (in picomoles) spotted is indicated next to each row.
Lane 1: unmodified Lys64 peptide.
Lane 2: monomethyl Lys64 peptide.
Lane 3: dimethyl Lys64 peptide.
Lane 4: trimethyl Lys64 peptide.
Lane 5: monomethyl Lys56 peptide.
Lane 6: dimethyl Lys56 peptide.
Lane 7: trimethyl Lys56 peptide.
Lane 8: monomethyl Lys79 peptide.
Lane 9: dimethyl Lys79 peptide.
Lane 10: trimethyl Lys79 peptide.
No detection of peptides (unmodified, mono-, di-, or tri-methylated) corresponding to lysine 4, lysine 9, lysine 18, lysine 23, lysine 27 and lysine 36 of Histone H3 was observed with Histone H3 dimethyl Lys79 antibody.
Histone H3 monomethyl Lys79 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µg of Histone H3 monomethyl Lys79 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
for Histone H3 monomethyl Lys79 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 monomethyl Lys79 antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
