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Antibodies

Ago3 antibody (mAb)

Clone: 4B1-F6
Catalog No: 39787 Format: 100 µg $340 Buy Now
Catalog No: 39788 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG2a
Purification:Protein G Chromatography
Applications:IF, IP
Reactivity:Human
Molecular Weight:110 kDa

Applications Table

Western Blot Chromatin IP IF IP
— — Published.
See Application Notes below.		 Published.
See Application Notes below.

for Ago3 antibody (mAb) (Clone 4B1-F6)Background

Argonaute 3 (Ago3, aka EIF2C3) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago3, however, lacks RNase activity and does not appear to cleave target mRNA molecules. Ago3 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins.

for Ago3 antibody (mAb) (Clone 4B1-F6)Immunogen

This Ago3 (Argonaute 3) antibody was raised against a recombinant protein corresponding to amino acids 1-175 of human Ago3.

for Ago3 antibody (mAb) (Clone 4B1-F6)Application Notes

This Ago3 (Argonaute 3) antibody has been validated for use in immunoprecipitation and immunofluorescence. Please see reference Azuma et al 2008 for more details.

for Ago3 antibody (mAb) (Clone 4B1-F6)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Ago3 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Ago3 antibody (mAb).

for Ago3 antibody (mAb) (Clone 4B1-F6)References

This antibody was used in immunoprecipitation and immunofluorescence as described:

Azuma-Mukai et al (2008) PNAS 105: 7964–7969

for Ago3 antibody (mAb) (Clone 4B1-F6)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Ago3 antibody (mAb) (Clone 4B1-F6)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting