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Recombinant Proteins & Enzymes

Recombinant SETDB1 protein

Aliases: SET Domain Bifurcated 1, ERG-Associated Protein With SET Domain, Histone H3-K9 Methyltransferase 4
Catalog No: 31452 Format: 20 µg $425 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant SETDB1 proteinContents

Recombinant SETDB1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SETDB1 proteinBackground

SETDB1 (SET Domain, Bifurcated 1) is a histone methyltransferase that methylates histone H3 at lysine 9. H3K9 methylation is associated with transcriptional repression and gene silencing through recruitment of HP1 proteins to methylated histones. H3K9 trimethylation is coordinated with DNA methylation, most likely through formation of a complex with MBD1 and ATF7IP that leads to transcriptional repression. SETDB1 activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28, a factor recruited by KRAB zinc-finger proteins. Aberrant SETDB1 function is associated with the pathogenesis of Huntington Disease.

for Recombinant SETDB1 proteinApplication Notes

Recombinant SETDB1 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 3 µM H3 (1-21) peptide was incubated with different concentrations of recombinant SETDB1 protein in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM for 3 hours at room temperature. Activity was detected by HTRF.

for Recombinant SETDB1 proteinProtein Details

Recombinant SETDB1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001138887.1) with an N-terminal FLAG-Tag. The molecular weight of the protein is 145 kDa.

 
Recombinant SETDB1 protein gel

Recombinant SETDB1 protein gel.
SETDB1 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.

HTRF Assay for Recombinant SETDB1 protein activity.

HTRF assay for Recombinant SETDB1 protein activity.
3 µM histone peptide H3 (1-21) was incubated with SETDB1 in reaction buffer including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 µM SAM for 3 hours at room temperature. Anti-H3K9me2 antibody was used to detect reaction product.

HTRF Assay for Recombinant SETDB1 protein activity.

HTRF assay for Recombinant SETDB1 protein activity.
3 µM histone peptide H3 (1-21) was incubated with SETDB1 in reaction buffer including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 µM SAM for 3 hours at room temperature. Anti-H3K9me2 antibody was used to detect reaction product.

for Recombinant SETDB1 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SETDB1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant SETDB1 protein
 

Data Thumbnails

Recombinant SETDB1 protein gel

Recombinant SETDB1 protein gel.
(Click image to enlarge and see details).

HTRF Assay for Recombinant SETDB1 protein activity.

HTRF Assay for Recombinant SETDB1 protein activity.
(Click image to enlarge and see details).

HTRF Assay for Recombinant SETDB1 protein activity.

HTRF Assay for Recombinant SETDB1 protein activity.
(Click image to enlarge and see details).