Ago1 antibody (mAb)

for Ago1 antibody (mAb) (Clone 4B8)Background

The Argonaute family proteins (Ago1, Ago2, Ago3 and Ago4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Argonaute family proteins contain a PAZ and a PIWI domain.

for Ago1 antibody (mAb) (Clone 4B8)Immunogen

This Ago1 antibody was raised against a peptide from the N-terminus of human Argonaute 1 (Ago1) protein.

for Ago1 antibody (mAb) (Clone 4B8)Application Notes

This Ago1 antibody has been validated for use in Western blotting and immunoprecipitation. See References for more details.

for Ago1 antibody (mAb) (Clone 4B8)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for Ago1 antibody (mAb) (Clone 4B8)References

This antibody has been used in the following references:
IP: Beltzinger M et al (2007) RNA Biol 4:76-84.
WB: Hock J et al (2007) EMBO Reports 8:1052-1060.

for Ago1 antibody (mAb) (Clone 4B8)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Ago1 antibody (mAb) (Clone 4B8)Guarantee

This product is guaranteed for 6 months from date of arrival.