5-Azacytidine
Aliases: 4-Amino-1-β-D-ribofuranosyl-1,3,5-triazin-2(1H)-one Ladakamycin; U-18496; NSC-102816; 5-AzaC |
Catalog No: 14102 | Format: 250 mg | $100 | Add to Cart |
Catalog No: 14103 | Format: 50 mg | $50 | Add to Cart |
Application: DNA Methylation |
Chemical Properties
MW = 244.2
C8H12N4O5
CAS 320-67-2
MP = 226-232°C (dec.)
>98% (TLC); NMR (Conforms)
Description
Specific and potent inhibitor of DNA methyltransferase, DNMT1 (ref 1). Acts as a suicide substrate for methyltransferases after incorporation into DNA (ref 1). Induces apoptosis in HCT-116 colon cancer cells which is mediated by p53 and its downstream effectors p21 (WAF1) and GADD45 (ref 2). Increases stem cell reprogramming efficiency (ref 3). Induces cardiac differentiation of human umbilical cord-derived MSCs (ref 4). Reverses drug resistance in bladder cancer cells (ref 5). Antileukemic agent (ref 6). Cell permeable.
Contents
White powder. May be dissolved in DMSO (25 mg/ml); Water (12 mg/ml).
Storage Conditions
Store desiccated as supplied at -20°C for up to 2 years. Store solutions at -20°C for up to 1 month.
References
1. C Stresemann and F Lyko Int. J. Cancer 2008, 123:8
2. R Schneider-Stock et al. J. Pharmacol. Exp. Ther. 2005, 312:525
3. TS Mikkelsen et al. Nature 2008 454:49
4. Q Qian et al. Stem Cells Dev. 2012, 21:67
5. K Ramachandran et al. Anticancer Res. 2011, 31:3757
6. J-PJ Issa et al. Clin. Cancer Res. 2009, 15:3939
Chemical structure of 5-Azacytidine.