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Recombinant Transposase Enzymes Overview
The recombinant Tn5 and pA-Tn5 enzymes are useful for many applications, including ATAC-Seq, CUT&Tag assays, Tagmentation, ChIPmentation, and more.
In addition to our ATAC-Seq Kits, bulk ATAC-Seq service, and single-cell ATAC-Seq service, CUT&Tag-IT Assay Kits, and CUT&Tag Service, Active Motif also offers highly active and pure recombinant Tn5 or pA-Tn5 enzymes.
Click the “get a quote” button to fill out a form to provide details about your bulk request, and your Key Account Manager will be in touch with ordering information.
Tn5 Overview
Each lot of our Recombinant Tn5 Transposase is QC tested by SDS-PAGE to show a protein band at ~55 kDa with >90% purity (Figure 1), and by a Tagmentation Test on genomic K562 cell DNA (Figure 2) to confirm that a majority of DNA is observed at 200 – 400 bp with an average sample intensity >1000 by analysis on Agilent TapeStation.
We use our Recombinant Tn5 Transposase in the Assembled Transposomes Component in the ATAC-Seq Assay Kits, and in our bulk ATAC-Seq Services.
pA-Tn5 Overview
Each lot of our Recombinant pA-Tn5 is QC tested by SDS-PAGE to show a protein band at ~73 kDa with >90% purity (Figure 3), and by a Tagmentation Test on genomic K562 cell DNA (Figure 4) to confirm that a majority of DNA is observed at 200 – 400 bp with an average sample intensity >1000 by analysis on Agilent TapeStation.
We use our pA-Tn5 in the CUT&Tag-IT™ Assembled Transposomes Component in the CUT&Tag-IT™ Assay Kits, and in our CUT&Tag-IT™ Services.
Recombinant Transposase Enzymes Data
Tn5 SDS-PAGE
Figure 1: 12.5% SDS PAGE Gel of Recombinant Tn5 Transposase Protein (MW 53kDa).
Tn5 Tagmentation Test
2A
2BFigure 2: Typical tagmentation profile of K562 genomic DNA after incubation with the Recombinant Tn5 Transposase.
50 ng of K562 genomic DNA was incubated for one hour with loaded Tn5 Transposase and ATAC Tagmentation Buffer. AMPure bead clean up was performed on the samples and was followed by Q5 PCR amplification. After another AMPure bead clean up, samples were analyzed for fragment size (gel image 2A) and electropherogram (2B) on the Agilent TapeStation.
pA-Tn5 SDS PAGE Gel
Figure 3: 12.5% SDS PAGE Gel of Recombinant pA-Tn5 Transposase Protein (MW 72kDa).
pA-Tn5 Tagmentation Test
4A
4BFigure 4: Typical tagmentation profile of K562 genomic DNA after incubation with the Recombinant pA-Tn5 Transposase.
50 ng of K562 genomic DNA was incubated for one hour with loaded pA-Tn5 Transposase and ATAC Tagmentation Buffer. AMPure bead clean up was performed on the samples and was followed by Q5 PCR amplification. After another AMPure bead clean up, samples were analyzed for fragment size (gel image 4A) and electropherogram (4B) on the Agilent TapeStation.
Recombinant Transposase Enzymes Documents
