Active Motif Webinars

Traditionally, Histone PTM levels are measured by western blot or ELISA but these methods require larger amounts of material and can only examine one modification at a time.

In this Webinar, Mary Anne Jelinek Ph.D., Senior Research Scientist at Active Motif will discuss our Histone H3 PTM Multiplex Assay and its advantages over traditional western blotting.

Although ChIP-Seq has contributed greatly to our understanding of the epigenome and gene regulation, it is not without its limitations, which can impede our ability to answer complex scientific questions.

In this Webinar, Adam Blattler Ph.D., Research Scientist at Active Motif will discuss the variety of tools and services we have developed to overcome many of these challenges.

Errors in gene expression are common in various human diseases. Accordingly, a thorough understanding of the mechanisms regulating or mis-regulating gene expression is crucial to understanding a diseases’ initiation and progression. Historically, ChIP-Seq and ChIP-Chip were the predominant methods used to analyze DNA/protein interactions, however, these methods can have limited resolution in identifying a transcription factor binding site.

In this Webinar, Bryce Alves, Ph.D. Research Scientist at Active Motif will discuss ChIP-exo, a powerful technique for high-resolution mapping of transcription factor binding sites.

An ongoing problem in ChIP-Seq data analysis is the often large discrepancy between expected changes in histone marks and the actual ChIP-Seq data when using drug treated cells.

In this Webinar, Brian Egan, Ph.D. Epigenetics Services Manager at Active Motif discusses a novel ChIP-Seq spike-in strategy and shows how normalization of ChIP-Seq data using this spike-in strategy reveals the expected changes in the final ChIP-Seq data set for inhibitor treated samples.

Chromatin Immunoprecipitation (ChIP) is a powerful tool for studying protein/DNA interactions, but it can be technically challenging. To date, ChIP studies have been limited mainly to cultured cells and model systems.

Join us for a Webinar during which Johanna Samuelsson, Ph.D. Sr. Research Scientist at Active Motif discusses the pitfalls & challenges of generating high quality ChIP data from difficult-to-ChIP samples, including primary cells, FFPE samples and PBMCs.

Here we describe a method using the LightSwitch Luciferase Assay System for the co-transfection of individual 3'UTR luciferase reporter constructs with a miRNA mimic. This protocol is efficient, reproducible, and amenable to high-throughput analysis.