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A Universal Spike-In Normalization Strategy for CUT&RUN, CUT&Tag, and ATAC-Seq
This poster was presented at AACR 2024, describing the development of unique reagents for assay normalization. The use of Spike-In controls as a means of normalization in NGS based assays is shown to be not only useful, but in some cases necessary to detect true differences in gene expression or protein occupancy. Data is presented comparing Spike-In normalization with techniques using Drosophila nuclei.
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