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ChIP Kit Selection Guide

Whether you are starting with limited amounts or difficult samples, need to begin by profiling open chromatin regions and histone marks, or are ready to focus on a specific transcription factor, the ChIP Selection Guides below can help you choose the products that are right for your experimental situation. Clicking on each image will open a larger version.

What is your source material?

Jump To: Cultured Cells | Frozen/FFPE Tissue or PBMCs | Profile Open Chromatin Regions | Limited Sample Material​

Cultured Cells

ChIP Kits Guide 1

* Note: Enzymatic shearing is inherently biased. Sonication is the preferred shearing method, if possible.

 

Frozen/FFPE Tissue or PBMCs

ChIP Kits Guide 2

Profile Open Chromatin Regions

Need to analyze open chromatin regions at genome-wide scale?

Profile Open Chromatin Regions Decision Tree

Limited Sample Material​

Limited Sample Material Decision Tree

What’s your sample input and target situation?

Limited amounts of cells​

Low Cell ChIP-Seq Kit enables transcription factor ChIP in limited samples from cell sorting, or sample segmentation for multi-omics processing. To profile histone modifications, CUT&Tag-IT™ Assay Kit generates results without a library prep step from as few as 5000 cells.​

Low abundance transcription factors​

ChIP-IT® High Sensitivity Kit is designed to enrich for low abundance transcription factors.​

One day protocol for ChIP​​

ChIP-IT® Express has the optimized streamlined workflow to get ChIP results in one day.​

No antibody exists for the target of interest​​

If ChIP-validated antibodies are not available for the target protein, the Tag-ChIP-IT® system works by creating an expression construct containing the protein of interest in-frame with a C-terminal AM-tag that is specifically designed for ChIP.​

Fomalin-fixed paraffin-embedded samples​​

The ChIP-IT™ FFPE II Kit is specifically designed to work with chromatin prep kits for both difficult-to-extract and readily extractable formalin-fixed paraffin-embedded (FFPE) samples.​

Peripheral blood mononuclear cell samples

The ChIP-IT® PBMC Kit is optimized with buffers to lyse peripheral blood mononuclear cells (PBMCs) and deliver high quality ChIP data.

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