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ChIP Kit Selection Guide
Whether you are starting with limited amounts or difficult samples, need to begin by profiling open chromatin regions and histone marks, or are ready to focus on a specific transcription factor, the ChIP Selection Guides below can help you choose the products that are right for your experimental situation. Click here to browse our complete offering of ChIP kit products.
What is your source material?
Jump To: Cultured Cells | Frozen Tissue or PBMCs | Profile Open Chromatin Regions | Limited Sample Material
Cultured Cells
| 1) Sample Type | 2) Do you have a ChIP, TIP-ChIP, or CUT&RUN validated antibody? |
3) Input Quantity Required | 4) Shearing | 5) Most Important Feature | 6) Recommended Kit |
|---|---|---|---|---|---|
| Cultured Cells | ⇨Yes, CUT&RUN | ⇨TFs: 25-500K Histones: 5-500K |
⇨Targeted Mnase Cleavage | ⇨2 Day Protocol | ⇨CUT&RUN #53180 |
| ⇨Yes, ChIP | ⇨TFs: 50-100K Histones: 1-100K |
⇨Sonication | ⇨Low Input Samples | ⇨Low Cell ChIP #53086 |
|
| ⇨100K+ | ⇨Sonication | ⇨Low Abundance Proteins/TFs | ⇨ChIP-IT High Sensitivity #53040 |
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| ⇨1 Day Protocol | ⇨ChIP-IT Express #53008 |
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| ⇨Enzymatic | ⇨1 Day Protocol | ⇨ChIP-IT Express Enzymatic #53009 |
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| ⇨Yes, ChIP, TIP-ChIP | ⇨300K - 1M per well | ⇨Tagmentation | ⇨Barcoded Multi-Sample ChIP | ⇨TIP-ChIP Assay Kit #53211 |
|
| ⇨No | ⇨6M | ⇨Sonication | ⇨No ChIP-Validated Antibody Required | ⇨Tag-ChIP-IT #53022 |
Frozen Tissue or PBMCs
| 1) Sample Type | 2) Input Quantity Required | 3) Most Important Feature | 4) Recommended Kit |
|---|---|---|---|
| Fresh/Frozen Tissue | ⇨TFs:10-20 mg Histones: 0.2-20 mg |
⇨Low Input Samples | ⇨Low Cell ChIP #53086 |
| ⇨100+ mg | ⇨Low Abundance Proteins/TFs | ⇨ChIP-IT High Sensitivity #53040 |
|
| ⇨1 Day Protocol | ⇨ChIP-IT Express #53008 |
||
| PBMCs | ⇨10M Cells | ⇨PBMC Compatibility | ⇨ChIP-IT PBMC #53042 |
Profile Open Chromatin Regions
| 1) Sample Type | 2) Input Quantity Required | 3) Most Important Feature | 4) Recommended Kit |
|---|---|---|---|
| Cultured Cells | ⇨1-100k | ⇨Integrated Library Preparation | ⇨ATAC-Seq Express Kit #53157 |
| Tissue (fresh or flash frozen) | ⇨20-30mg | ⇨Integrated Library Preparation | |
| Fixed Cells | ⇨50-100K | ⇨Formaldehyde-fixed Cells | ⇨Fixed Cell ATAC-Seq Kit #53151 |
Limited Sample Material
| 1) Sample Type | 2) Profiling Protein of Interest | 3) Input Quantity Required | 4) Most Important Feature | 5) Sequencing Depth Required | 6) Recommended Kit |
|---|---|---|---|---|---|
| Culture Cells | ⇨TFs or Histones | ⇨5-500K | ⇨Targeted MNase Cleavage | ⇨8-25 Million Reads | ⇨CUT&RUN #53180 |
| ⇨TFs | ⇨50-100K | ⇨Low Input | ⇨20-50 Million Reads | ⇨Low Cell ChIP #53086 |
|
| ⇨Histones | ⇨5-100K | ⇨Integrated Library Prep Step | ⇨8-10 Million Reads | ⇨CUT&Tag-IT Express #53175 |
|
| Tissue | ⇨Histones | ⇨0.5-10mg | ⇨Integrated Library Prep Step | ⇨8-10 Million Reads | ⇨CUT&Tag-IT Express #53175 Tissue Prep for NGS Assays #53185 |
Find the best ChIP kit for your sample input and target situation
If your sample input is limited, you can still perform high-quality ChIP or chromatin profiling using optimized kits:
- Low Cell ChIP Kit — designed for transcription factor ChIP from small or rare samples such as sorted cells or segmented populations for multi-omics workflows.
- CUT&Tag-IT® Express Assay Kit — ideal for profiling histone modifications from as few as 5,000 cells, without requiring a library preparation step.
Both options provide reliable results with low background and are suited for researchers working with rare cell populations or small sample volumes.
The ChIP-IT® High Sensitivity Kit is specifically optimized to detect and enrich low abundance transcription factors. Its enhanced chromatin preparation and antibody binding conditions enable reliable ChIP results even from limited or weakly expressed targets.
The ChIP-IT® Express Kit provides a streamlined, one-day workflow for ChIP experiments. This optimized protocol reduces hands-on time and processing steps while maintaining high yield and reproducibility.
Ideal for researchers needing fast turnaround and consistent results, the ChIP-IT Express Kit enables chromatin preparation, immunoprecipitation, and DNA purification all in a single day.
If a ChIP-validated antibody is not available, the Tag-ChIP-IT® system enables ChIP of any protein by expressing it with a C-terminal AM-tag. This tag is specifically designed for efficient and reliable ChIP, allowing researchers to study proteins that lack suitable antibodies.
Yes. The ChIP-IT® PBMC Kit is specifically formulated with optimized buffers to efficiently lyse peripheral blood mononuclear cells (PBMCs) and provide high-quality ChIP data from these primary cell types.
