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CUT&Tag (Cleavage Under Targets and Tagmentation) utilizes a protein A (pA) Tn5 chimera (pA-Tn5) and a mouse or rabbit primary antibody to a histone or histone mark of interest to tether the pA-Tn5. This directs the Tn5 activity to tagment the DNA around the target of interest genome-wide, without any NGS library preparation steps, to reveal the genetic sequences of those sites of interest. CUT&Tag requires much less input material and sequencing read depths than ChIP-Seq, and can be perfomed in 1-2 days.
Profile histone marks with rabbit or mouse antibodies from as few as 5,000 cells.
Profile histone marks with rabbit or mouse antibodies from 0.5 to 10 mg of tissue.
