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Some isotypes of mouse antibody do not bind well to the protein G-conjugated agarose/magnetic beads used in standard immunoprecipitation and chromatin immunoprecipitation experiments. This can be improved by the inclusion of an anti-mouse IgG bridging antibody, which binds with a strong affinity to both the protein G beads and the mouse primary antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, use of the Bridging Antibody for Mouse IgG helps improve the results of all ChIP and IP experiments that use mouse primary antibody.
| Name | Format | Cat No. | Price | |
|---|---|---|---|---|
| Bridging Antibody for Mouse IgG | 500 µg | 53017 | $130 | Add to Cart |
| Bridging Antibody for Mouse IgG Data Sheet |
| Active Motif Antibodies Brochure |
| Epigenetics Products and Services |
| MSDS: Sodium Azide |
The Bridging Antibody for Mouse IgG helps improve immunoprecipitation (ChIP and IP) experiments that use primary mouse antibody by improving the affinity of the mouse primary antibody for the protein G-conjugated agarose/magnetic beads that are used in immunoprecipitation (Figure 1).
Figure 1: Improvement in ChIP using the Bridging Antibody for Mouse IgG.
ChIP was performed using chromatin from U-937 cells induced with TNF-α (10 ng/ml for one hour). PCR was performed with primers corresponding to the human IL-8 promoter.
Lanes 1-4: Beads pre-incubated with no bridging antibody.
Lanes 5-8: Beads pre-incubated with 5 µg bridging antibody.
Lanes 1, 2, 5 & 6: ChIP performed using NFκB p65 mouse monoclonal antibody, 2 µg per IP.
Lanes 3, 4, 7 & 8: ChIP performed using negative control mouse IgG.
Contents & Storage
Five hundred µg of affinity-purified anti-mouse IgG in PBS, 30% glycerol and 0.035% sodium azide. Store at -20°C. Guaranteed stable for 6 months when stored properly.
