PIXUL™ Multi-Sample Sonicator

Upgrade to PIXUL to achieve truly reproducible shearing of chromatin, DNA, RNA, and protein.

 

PIXUL Multi-Sample Sonicator Overview

Next-generation sequencing applications, such as ChIP-Seq, RNA-Seq, RIP-Seq, exome sequencing, and whole genome sequencing require precise sample fragmentation (200-600 bp) for compatibility with short read sequencing platforms. Shearing by physical sonication, as opposed to enzymatic methods, is preferred for its unbiased nature and ability to produce a relatively narrow fragmentation profile.

However, most currently available sonication systems are incompatible with high-throughput sample processing or require expensive proprietary plates or tubes. PIXUL is the first and only multi-sample sonicator that delivers extremely consistent shearing of up to 96 samples processed in parallel and is fast, simple, and inexpensive to operate.

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PIXUL Multi-Sample Sonicator Highlights:

Consistent

Consistent: Process 1-96 samples simultaneously with extremely high reproducibility.


Simple

Simple: Fast setup, short learning curve, and easy operation with intuitive touchscreen.


Fast

Fast: Arrayed transducers and no degassing required saves time.


Flexible

Flexible: Use up to 12 different sonication conditions per run in 96-well plates for simultaneous processing of DNA, RNA, and protein sample types.


Affordable

Affordable: No requirement for buying accessories or expensive proprietary plates or tubes.

What Makes PIXUL Different

Advanced Sonication with the PIXUL Multi-Sample Sonicator

Watch this short video to learn more about PIXUL, the first and only multi-sample sonicator that delivers extremely consistent shearing of up to 96 samples processed in parallel and is fast, simple, and inexpensive to operate.

PIXUL sonication comparison

Unsurpassed Sonication Consistency at an Affordable Operating Cost

High-throughput sonication on other platforms is currently limited by the cost of consumables (expensive proprietary tubes, plates, and reagents). However, the low cost, round-bottom plates used with the PIXUL Multi-Sample Sonicator are directly compatible with cell culture and shearing, eliminating sample transfer steps that are inconvenient and can lead to sample loss. Furthermore, the Coupling Fluid used in the PIXUL instrument does not require lengthy degassing and can be adequately circulated and ready for sonication in approximately 15 minutes. The sonication run itself for up to 96 samples takes just 10-30 minutes, depending on the sample type and application.

Comparison of Pricing, Throughput, and Workflow Preparation Time for PIXUL and Competitor Sonication Systems

What our customers are saying about the PIXUL Multi-Sample Sonicator:

"When I used the PIXUL Multi-Sample Sonicator at The London BioFoundry, I found that it was very easy to use and maintain. I have confidence in PIXUL’s ability to perform reproducible sonication and I envision the successful application of the PIXUL system towards our ongoing synthetic biology applications."

Dr. Richard Kelwick
Research Associate
Dept. Infectious Disease
Section of Structural and Synthetic Biology
Imperial College London

"I used the PIXUL Multi-Sample Sonicator in my experiments and I was impressed by its ease of use, efficacy, and reproducibility."

Dr. Matthew Haines
Research Associate
Dept. Infectious Disease
Section of Structural and Synthetic Biology
Imperial College London

   

Dr. David Gorkin Discusses the PIXUL

Dr. David Gorkin, the Associate Director of Epigenomics at the Center for Epigenomics at UCSD, discusses his experiencing demoing our PIXUL Multi-Sample Sonicator and covers its advantages relative to other sonication instruments.

 

PIXUL Multi-Sample Sonicator Data

Extremely Consistent Shearing of Genomic DNA

Highly consistent shearing of genomic DNA to an appropriate size for conversion into functional NGS libraries is integral to high quality and comparable next-generation sequencing data. Manual shearing with a probe sonicator or shearing with an inconsistent high-throughput sonicator can lead to sample-to-sample variability.

To test the consistency of the PIXUL Multi-Sample Sonicator, purified genomic DNA was loaded into all 96 wells of the PIXUL 96-well plate, processed, and fragmentation profiles examined for sonication consistency and efficiency. Highly consistent average fragment lengths and standard deviations were observed across the entire plate.

DNA Shearing Consistency with the PIXUL Instrument

Figure 1: The PIXUL Multi-Sample Sonicator consistently sonicates purified genomic DNA samples to an optimal fragmentation profile. 10 µg of salmon sperm DNA was loaded into each well of the PIXUL 96-well plate. The samples were processed for 36 minutes. Fragmentation profiles were examined on an agarose gel. Average fragment size is 307 +/- 35 bp. Nucleic Acids Res. 2019 Jul 9; 47(12):e69.

PIXUL Delivers More Consistent Chromatin Shearing than Competitor C

ChIP-Seq studies benefit from relatively narrow and consistent chromatin shearing profiles, as this can improve the resolution of transcription factor binding sites or the locations of histone post-translational modifications.

To evaluate chromatin sonication consistency between different multi-sample sonicators, we compared chromatin shearing performed with the PIXUL to a leading Competitor C sonication system. PIXUL demonstrated more consistency in chromatin shearing than the Competitor C system.

Chromatin Shearing Consistency with the PIXUL Compared to Competitor C

Figure 2: The PIXUL Multi-Sample Sonicator consistently sonicates chromatin to an optimal fragmentation profile. 200,000 HCT116 cells were loaded into each well of the PIXUL 96-well plate or the proprietary Competitor C plate. Sonication of these samples was performed and fragmentation profiles were examined by agarose gel electrophoresis. PIXUL samples have a tighter size distribution and increased consistency across the plate. Nucleic Acids Res. 2019 Jul 9; 47(12):e69.

PIXUL Enables More Consistent Sonication and Higher Chromatin Recovery than Competitor D

ChIP-Seq studies have historically required chromatin preparation from millions of cells because the limitations created by inefficienct sonication. As ChIP-Seq techniques have progressed, the number of input cells required has decreased by maximizing the efficiency of sonication and chromatin recovery.

PIXUL 96-well plates are compatible with cell culture, fixation, and sonication steps there's no need to transfer samples from culture plates to sonication plates and risk sample loss. Compared to Competitor D, PIXUL exhibits a tighter size distribution of chromatin fragments following sonication, as well as higher chromatin recovery (data not shown).

Chromatin Shearing is More Consistent with PIXUL than Competitor D

Figure 3: The PIXUL Multi-Sample Sonicator consistently sonicates chromatin to an optimal fragmentation profile. HEK293 cells were loaded into each well of the PIXUL 96-well plate. They were cultured, fixed, and chromatin fragmented in the same plate without any sample transfers. Competitor D exhibits less consistent fragmentation. Nucleic Acids Res. 2019 Jul 9; 47(12):e69.

PIXUL Delivers Equivalent Exome Sequencing Results as Competitor C for a Fraction of the Cost

PIXUL was compared to Competitor C in an exome sequencing experiment, which requires DNA shearing as a first step. Using 500 ng of input DNA, samples were sheared with either PIXUL or Competitor C, libraries were constructed, enriched for the exome, and sequenced. Several sequencing metrics were analyzed to determine the PIXUL instrument produced exome libraries that performed equivalently to Competitor C, at just a fraction of the sample preparation cost for the instrument and consumables. 

Exome Sequencing Metrics from PIXUL and Competitor C

Figure 4: The PIXUL and Competitor C sonication systems were used to shear 500 ng genomic DNA samples prior to constructing exome sequencing libraries. NGS metrics indicate that these libraries performed equivalently. Nucleic Acids Res. 2019 Jul 9; 47(12):e69.

     
 

PIXUL Multi-Sample Sonicator Storage & Contents

The PIXUL Multi-Sample Sonicator is shipped at room temperature and should be operated in an environment of 15°C - 25°C and maximum relative humidity of 85%.

Each unit includes the following components:

  • PIXUL instrument
  • Power cord
  • Drain hose
 

PIXUL Multi-Sample Sonicator Documents

 


 

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You might also be interested in:

PIXUL™ is sold under an exclusive license to patents owned by Matchstick Technologies Inc. and University of Washington, specifically Matula, T.J.; Bomsztyk, K.; Darlington, G.P., Maxwell, A.D.; MacConaghy, B.E., Reed, J. “Ultrasound system for shearing cellular material”. US Patent US10809166. European Patent EP3169451.

 
Name Format Cat No. Price  
PIXUL™ Multi-Sample Sonicator 1 unit 53130 Get Quote
PIXUL™ gDNA Shearing Kit 1 x 96 rxns 53131 $495 Buy
PIXUL™ Chromatin Shearing Kit 1 x 96 rxns 53132 $295 Buy
PIXUL™ Chromatin Input Preparation Kit 96 rxns 53134 $295 Buy
PIXUL™ Coupling Fluid 1 L 53136 $225 Buy
PIXUL™ 96-well Plate with Sealer 1 unit 53139 $25 Buy