PIXUL™ Multi-Sample Sonicator
Upgrade to PIXUL to achieve truly reproducible shearing of chromatin, DNA, RNA, and protein.
PIXUL Multi-Sample Sonicator Overview
PIXUL Multi-Sample Sonicator Highlights:
Unsurpassed Sonication Consistency at an Affordable Operating Cost
High-throughput sonication on other platforms is currently limited by the cost of consumables (expensive proprietary tubes, plates, and reagents). However, the low cost, round-bottom plates used with the PIXUL Multi-Sample Sonicator are directly compatible with cell culture and shearing, eliminating sample transfer steps that are inconvenient and can lead to sample loss. Furthermore, the Coupling Fluid used in the PIXUL instrument does not require lengthy degassing and can be adequately circulated and ready for sonication in approximately 15 minutes. The sonication run itself for up to 96 samples takes just 10-30 minutes, depending on the sample type and application.
PIXUL Multi-Sample Sonicator Data
Extremely Consistent Shearing of Genomic DNA
Highly consistent shearing of genomic DNA to an appropriate size for conversion into functional NGS libraries is integral to high quality and comparable next-generation sequencing data. Manual shearing with a probe sonicator or shearing with an inconsistent high-throughput sonicator can lead to sample-to-sample variability.
To test the consistency of the PIXUL Multi-Sample Sonicator, purified genomic DNA was loaded into all 96 wells of the PIXUL 96-well plate, processed, and fragmentation profiles examined for sonication consistency and efficiency. Highly consistent average fragment lengths and standard deviations were observed across the entire plate.
PIXUL Delivers More Consistent Chromatin Shearing than Competitor C
ChIP-Seq studies benefit from relatively narrow and consistent chromatin shearing profiles, as this can improve the resolution of transcription factor binding sites or the locations of histone post-translational modifications.
To evaluate chromatin sonication consistency between different multi-sample sonicators, we compared chromatin shearing performed with the PIXUL to a leading Competitor C sonication system. PIXUL demonstrated more consistency in chromatin shearing than the Competitor C system.
PIXUL Enables More Consistent Sonication and Higher Chromatin Recovery than Competitor D
ChIP-Seq studies have historically required chromatin preparation from millions of cells because the limitations created by inefficienct sonication. As ChIP-Seq techniques have progressed, the number of input cells required has decreased by maximizing the efficiency of sonication and chromatin recovery.
PIXUL 96-well plates are compatible with cell culture, fixation, and sonication steps there's no need to transfer samples from culture plates to sonication plates and risk sample loss. Compared to Competitor D, PIXUL exhibits a tighter size distribution of chromatin fragments following sonication, as well as higher chromatin recovery (data not shown).
PIXUL Delivers Equivalent Exome Sequencing Results as Competitor C for a Fraction of the Cost
PIXUL was compared to Competitor C in an exome sequencing experiment, which requires DNA shearing as a first step. Using 500 ng of input DNA, samples were sheared with either PIXUL or Competitor C, libraries were constructed, enriched for the exome, and sequenced. Several sequencing metrics were analyzed to determine the PIXUL instrument produced exome libraries that performed equivalently to Competitor C, at just a fraction of the sample preparation cost for the instrument and consumables.
PIXUL Multi-Sample Sonicator Storage & Contents
The PIXUL Multi-Sample Sonicator is shipped at room temperature and should be operated in an environment of 15°C - 25°C and maximum relative humidity of 85%.
Each unit includes the following components:
- PIXUL instrument
- Power cord
- Drain hose
PIXUL Multi-sample Sonicator Documents
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PIXUL™ is sold under an exclusive license to patents owned by Matchstick Technologies Inc. and University of Washington, specifically Matula, T.J.; Bomsztyk, K.; Darlington, G.P., Maxwell, A.D.; MacConaghy, B.E., Reed, J. “Ultrasound system for shearing cellular material”. US Patent US10809166. European Patent EP3169451.
|PIXUL™ Multi-Sample Sonicator||1 unit||53130||Get Quote|
|PIXUL™ gDNA Shearing Kit||1 x 96 rxns||53131||$495||Buy|
|PIXUL™ Chromatin Shearing Kit||1 x 96 rxns||53132||$295||Buy|
|PIXUL™ Chromatin Input Preparation Kit||96 rxns||53134||$295||Buy|
|PIXUL™ Coupling Fluid||1 L||53136||$225||Buy|
|PIXUL™ 96-well Plate with Sealer||1 unit||53139||$25||Buy|