PIXUL Multi-Sample Sonicator Overview
PIXUL Kits: Consistent shearing performance for downstream NGS and ChIP assays with PIXUL gDNA and chromatin shearing kits.
PIXUL Labware: Sonication convenience with labware specifically designed for the PIXUL sonication workflow.
Next-generation sequencing applications, such as ChIP-Seq, RNA-Seq, RIP-Seq, exome sequencing, and whole genome sequencing require precise sample fragmentation (200-600 bp) for compatibility with short read sequencing platforms. Shearing by physical sonication, as opposed to enzymatic methods, is preferred for its unbiased nature and ability to produce a relatively narrow fragmentation profile.
However, most currently available sonication systems are incompatible with high-throughput sample processing or require expensive proprietary plates or tubes. PIXUL is the first and only multi-sample sonicator that delivers extremely consistent shearing of up to 96 samples processed in parallel and is fast, simple, and inexpensive to operate.
PIXUL Multi-Sample Sonicator Highlights:
Consistent: Process 1-96 samples simultaneously with extremely high reproducibility.
Simple: Fast setup, short learning curve, and easy operation with intuitive touchscreen.
Fast: Arrayed transducers and no degassing required saves time.
Flexible: Use up to 12 different sonication conditions per run in 96-well plates for simultaneous processing of DNA, RNA, and protein sample types.
Affordable: No requirement for buying accessories or expensive proprietary plates or tubes.
What Makes PIXUL Different
Advanced Sonication with the PIXUL Multi-Sample Sonicator
Watch this short video to learn more about PIXUL, the first and only multi-sample sonicator that delivers extremely consistent shearing of up to 96 samples processed in parallel and is fast, simple, and inexpensive to operate.
Unsurpassed Sonication Consistency at an Affordable Operating Cost
High-throughput sonication on other platforms is currently limited by the cost of consumables (expensive proprietary tubes, plates, and reagents). However, the low cost, round-bottom plates used with the PIXUL Multi-Sample Sonicator are directly compatible with cell culture and shearing, eliminating sample transfer steps that are inconvenient and can lead to sample loss. Furthermore, the Coupling Fluid used in the PIXUL instrument does not require lengthy degassing and can be adequately circulated and ready for sonication in approximately 15 minutes. The sonication run itself for up to 96 samples takes just 10-30 minutes, depending on the sample type and application.
What our customers are saying about the PIXUL Multi-Sample Sonicator:
"When I used the PIXUL Multi-Sample Sonicator at The London BioFoundry, I found that it was very easy to use and maintain. I have confidence in PIXUL’s ability to perform reproducible sonication and I envision the successful application of the PIXUL system towards our ongoing synthetic biology applications."
"I used the PIXUL Multi-Sample Sonicator in my experiments and I was impressed by its ease of use, efficacy, and reproducibility."
Dr. David Gorkin Discusses the PIXUL
Dr. David Gorkin, the Associate Director of Epigenomics at the Center for Epigenomics at UCSD, discusses his experiencing demoing our PIXUL Multi-Sample Sonicator and covers its advantages relative to other sonication instruments.
PIXUL Multi-Sample Sonicator Data
Extremely Consistent Shearing of Genomic DNA
Highly consistent shearing of genomic DNA to an appropriate size for conversion into functional NGS libraries is integral to high quality and comparable next-generation sequencing data. Manual shearing with a probe sonicator or shearing with an inconsistent high-throughput sonicator can lead to sample-to-sample variability.
To test the consistency of the PIXUL Multi-Sample Sonicator, purified genomic DNA was loaded into all 96 wells of the PIXUL 96-well plate, processed, and fragmentation profiles examined for sonication consistency and efficiency. Highly consistent average fragment lengths and standard deviations were observed across the entire plate.
For consistent gDNA shearing, we recommend the PIXUL gDNA Shearing Kit.
PIXUL Delivers More Consistent Chromatin Shearing than Competitor C
ChIP-Seq studies benefit from relatively narrow and consistent chromatin shearing profiles, as this can improve the resolution of transcription factor binding sites or the locations of histone post-translational modifications.
To evaluate chromatin sonication consistency between different multi-sample sonicators, we compared chromatin shearing performed with the PIXUL to a leading Competitor C sonication system. PIXUL demonstrated more consistency in chromatin shearing than the Competitor C system.
For consistent chromatin shearing, we recommend the PIXUL Chromatin Shearing Kit. The Chromatin Input Preparation Kit makes it easy to quantitate yield and fragmentation efficiency prior to ChIP.
PIXUL Enables More Consistent Sonication and Higher Chromatin Recovery than Competitor D
ChIP-Seq studies have historically required chromatin preparation from millions of cells because the limitations created by inefficienct sonication. As ChIP-Seq techniques have progressed, the number of input cells required has decreased by maximizing the efficiency of sonication and chromatin recovery.
PIXUL 96-well plates are compatible with cell culture, fixation, and sonication steps there's no need to transfer samples from culture plates to sonication plates and risk sample loss. Compared to Competitor D, PIXUL exhibits a tighter size distribution of chromatin fragments following sonication, as well as higher chromatin recovery (data not shown).
PIXUL Delivers Equivalent Exome Sequencing Results as Competitor C for a Fraction of the Cost
PIXUL was compared to Competitor C in an exome sequencing experiment, which requires DNA shearing as a first step. Using 500 ng of input DNA, samples were sheared with either PIXUL or Competitor C, libraries were constructed, enriched for the exome, and sequenced. Several sequencing metrics were analyzed to determine the PIXUL instrument produced exome libraries that performed equivalently to Competitor C, at just a fraction of the sample preparation cost for the instrument and consumables.
PIXUL Multi-Sample Sonicator Storage & Contents
The PIXUL Multi-Sample Sonicator is shipped at room temperature and should be operated in an environment of 15°C - 25°C and maximum relative humidity of 85%.
Each unit includes the following components:
- PIXUL instrument
- Power cord
- Drain hose
PIXUL Multi-Sample Sonicator Publications
Search our database of customer publications that have used our PIXUL Multi-Sample Sonicator.
PIXUL Multi-Sample Sonicator Documents
- PIXUL™ Multi-Sample Sonicator Manual (After Nov 2021)
- PIXUL™ Multi-Sample Sonicator Manual (Through Oct 2021)
TechNotes & Supplemental Protocols
- Supplemental Protocol: Yeast Lysis with PIXUL™ Multi-Sample Sonicator
- Supplemental Protocol: Bacterial Lysis with PIXUL™ Multi-Sample Sonicator
- TechNote: Sample Preparation for Proteomics Applications with PIXUL™
- TechNote: Sonicating Limited Tissue Samples with PIXUL™ Multi-Sample Sonicator for ChIP-Seq Analysis
- TechNote: Sonication Recommendations for ChIP
- Quick Guide: PIXUL™ Multi-Sample Sonicator
- Quick Guide: Shearing Chromatin from Frozen Tissues with PIXUL™
You might also be interested in:
- Request More Information or Schedule a PIXUL Demo
- PIXUL Kits
- PIXUL Labware
- ChIP-IT High Sensitivity® Kit
- Download ChIP 101 eBook
- Guide to Generating the Best ChIP Data Every Time
- ChIP-Seq Services
- ChIP Validated Anitbodies
PIXUL™ is sold under an exclusive license to patents owned by Matchstick Technologies Inc. and University of Washington, specifically Matula, T.J.; Bomsztyk, K.; Darlington, G.P., Maxwell, A.D.; MacConaghy, B.E., Reed, J. “Ultrasound system for shearing cellular material”. US Patent US10809166. European Patent EP3169451.
|PIXUL™ Multi-Sample Sonicator||1 unit||53130||Get Quote|
|PIXUL™ Coupling Fluid||1 L||53136||$235||Buy|
|PIXUL™ 96-well Plate with Sealer||1 unit||53139||$30||Buy|