c-Myc Toolbox

The c-Myc oncogene encodes a transcription factor whose broad effects trigger selective gene expression amplification to promote cell growth and proliferation. Myc is one of the top targets for the development of cancer therapeutic options.

Active Motif can support early stage c-Myc research, screening and compound mechanism-of-action studies with our range of c-Myc products and services.

c-Myc Binding Assay

c-Myc Trans AM

Product:  c-Myc TransAM®

Function: Measures Global c-Myc binding activity.

Starting Material: Nuclear Extract

Throughput: Mid

Notes:   Immobilized c-Myc E-box binding site used to bind and capture active c-Myc on a 96-stripwell plate. Nuclear extracts are prepared using Active Motif's Nuclear Extract Kit.

Cell-based c-Myc Assays

C-Myc Luciferase Assay System

Product:     LightSwitch™ Promoter Reporter Collection

Function: Screen cells for c-Myc activity

Starting Material: Cells

Throughput: High

Notes:   Both Synthetic c-Myc luciferase reporter vectors and pre-cloned reporter vectors with c-Myc responsive gene promoters (e.g. TP53 and TERT) are available.

c-Myc Antibodies & Recombinant Proteins

C-Myc Antibodies

Product:  c-Myc antibodies & Rec. Proteins

Function: Validated for WB, EMSA, & IP

Notes:   Validated to work in our TransAM and EMSA Kits.

Genome-wide c-Myc Profiles

C-Myc Services

Product:  End-to-end ChIP-Seq Services

Function: Identify c-Myc binding across all genomic locations

Starting Material: Cells

Notes:   End to end service, performed by our ChIP-Seq experts using our validated protocols and antibodies. Simply send us your frozen cells and we will send back analyzed data.

miRNA Regulation of c-Myc

Mouse

Product:   LightSwitch 3´UTR & miRNA Reporter Collections

Function: Measure miRNA regulation of c-Myc.

Starting Material: Cells

Throughput: High

Notes:   3´UTR reporter constructs are available for c-Myc, E2F, and other c-Myc pathway related genes. A large collection of miRNA mimics are also available including those known to be regulated by c-Myc or to interact with c-Myc transcripts (mir34a, mir451).