β-Galactosidase Staining Kit
fast, simple colorimetric assay
The β-Galactosidase Staining Kit is an easy-to-use and efficient method to determine the percentage of cells expressing lacZ following transient or stable transfection of plasmids that contain lacZ.
The gene product of lacZ, β-galactosidase, catalyzes the hydrolysis of X-gal, which produces a blue color that is easily visualized. The kit can also be used to determine the efficiency of direct delivery of β-galactosidase by the Chariot™ Protein Delivery Reagent (Figure 1). It is also suitable for assaying tissue sections after in vivo transfection.
Figure 1: Staining of HeLa cells using the β-Galactosidase Staining Kit.
Contents & Storage
Staining Solutions 1, 2 & 3, 10X PBS, 10X Fixing Solution and X-gal. Sufficient reagents are included to perform 75 assays in 35 mm plates. Reagent storage conditions vary from room temperature to –20°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.