Nrf2 Toolbox

Nrf2 is an important transcription factor (TF) in the defense against oxidative stress and has been linked to diseases such as multiple sclerosis, diabetes and cancer. Fortunately, Nrf2 agonists and inhibitors are being developed as therapeutic options.

Active Motif can support early stage Nrf2 research, screening and compound mechanism-of-action studies with our range of Nrf2 products and services.


Nrf2 Binding Assay

Nrf2 Trans AM

Product:  Nrf2 TransAM®

Function: Measures Global Nrf2 binding activity.

Starting Material: Nuclear Extract

Throughput: Mid

Notes:   Immobilized ARE used to bind and capture active Nrf2 on a 96-stripwell plate. Nuclear extracts are prepared using Active Motif's Nuclear Extract Kit


Cell-based Nrf2 Assays

Nrf2 Luciferase Assay System

Product:  LightSwitch™ Promoter Reporter Collection

Function: Screen cells for Nrf2 activity

Starting Material: Cells

Throughput: High

Notes:   Both Synthetic ARE-luciferase reporter vectors and pre-cloned reporter vectors with Nrf2 responsive gene promoters (e.g. NQO1 and HMOX1) are available.


Nrf2 Antibody

Nrf2 Antibodies

Product:   ChIP-Seq validated Nrf2 antibody

Function: Validated for ChIP-Seq & Western Blot

Notes:   Validated to work in ChIP and ChIP-Seq with our ChIP-IT® High-Sensitivity and ChIP-IT® ChIP-Seq Kits


Genome-wide Nrf2 Profiles

Nrf2 Services

Product:  End-to-end ChIP-Seq Services

Function: Identify Nrf2 binding across all genomic locations

Starting Material: Cells

Notes:   End to end service, performed by our ChIP-Seq experts using our validated protocols and antibodies. Simply send us your frozen cells and we will send back analyzed data.


miRNA Regulation of Nrf2

Nrf2 Luciferase Assay System

Product:  LightSwitch 3´UTR & miRNA Reporter Collections

Function: Measure miRNA regulation of Nrf2.

Starting Material: Cells

Throughput: High

Notes:   3´UTR reporter constructs are available for Nrf2, KEAP1, and other Nrf2 pathway related genes. A large collection of miRNA mimics are also available including those known to interact withNrf2 transcripts (mir153, mir28) as well as miRNA inhibitors that can be used to knock down endogenous miRNAs.