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Nrf2 Toolbox
Nrf2 is an important transcription factor (TF) in the defense against oxidative stress and has been linked to diseases such as multiple sclerosis, diabetes and cancer. Fortunately, Nrf2 agonists and inhibitors are being developed as therapeutic options.
Active Motif can support early stage Nrf2 research, screening and compound mechanism-of-action studies with our range of Nrf2 products and services.
Nrf2 Binding Assay
Product: Nrf2 TransAM®
Function: Measures Global Nrf2 binding activity.
Starting Material: Nuclear Extract
Throughput: Mid
Notes: Immobilized ARE used to bind and capture active Nrf2 on a 96-stripwell plate. Nuclear extracts are prepared using Active Motif's Nuclear Extract Kit
Cell-based Nrf2 Assays
Product: LightSwitch™ Promoter Reporter Collection
Function: Screen cells for Nrf2 activity
Starting Material: Cells
Throughput: High
Notes: Both Synthetic ARE-luciferase reporter vectors and pre-cloned reporter vectors with Nrf2 responsive gene promoters (e.g. NQO1 and HMOX1) are available.
Nrf2 Antibody
Product: ChIP-Seq validated Nrf2 antibody
Function: Validated for ChIP-Seq & Western Blot
Notes: Validated to work in ChIP and ChIP-Seq with our ChIP-IT® High-Sensitivity and ChIP-IT® ChIP-Seq Kits
Genome-wide Nrf2 Profiles
Product: End-to-end ChIP-Seq Services
Function: Identify Nrf2 binding across all genomic locations
Starting Material: Cells
Notes: End to end service, performed by our ChIP-Seq experts using our validated protocols and antibodies. Simply send us your frozen cells and we will send back analyzed data.
miRNA Regulation of Nrf2
Product: LightSwitch™ 3´UTR & miRNA Reporter Collections
Function: Measure miRNA regulation of Nrf2.
Starting Material: Cells
Throughput: High
