Recombinant c-Myc protein

Aliases: MRTL, MYCC, c-Myc, bHLHe39
Catalog No: 31117 Format: 5 µg $455 Buy
Expressed In: E. coli Protein Species: Human

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Contents

5 µg of Recombinant c-Myc protein in Dilution Buffer AM1 (20 mM Tris-Cl (pH 7.5), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Please refer to product insert upon arrival for lot-specific concentration.

Background

c-Myc (Myc) is a sequence specific DNA binding transcription factor that regulates gene expression, especially genes involved in cell division. Myc family members contain a basic Helix-Loop-Helix leucine zipper (bHLHZ) domain. Myc family proteins associated with thousands of genes, especially those involved in cell growth and proliferation. c-Myc serves as an integrator of signal transduction, linking extracellular signals to an acute cellular growth response. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.

What’s in our c-Myc Toolbox?

Application Notes

Recombinant c-Myc protein is suitable for DNA and protein-protein interaction assays. 100 ng are sufficient for DNA-protein and protein-protein interaction studies. The standard curve for TransAM® c-Myc was generated using the range of 5-0.625 ng of protein. The molecular weight of the protein is ~50 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

Protein Details

Recombinant c-Myc was expressed in a bacterial system (accession number NM 002467) with an N-terminal His-Tag and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 75% homogeneous and contains no detectable protease, DNase and RNase activity.

References


This product was used in the following publications:
Acta Biochim Pol. 2011;58(4):529-34. Epub 2011 Nov 8.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.