Recombinant c-Myc protein
|Aliases: MRTL, MYCC, c-Myc, bHLHe39|
|Catalog No: 31117||Format: 5 µg||$470||Buy|
Expressed In: E. coli Protein Species: Human
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
c-Myc (Myc) is a sequence specific DNA binding transcription factor that regulates gene expression, especially genes involved in cell division. Myc family members contain a basic Helix-Loop-Helix leucine zipper (bHLHZ) domain. Myc family proteins associated with thousands of genes, especially those involved in cell growth and proliferation. c-Myc serves as an integrator of signal transduction, linking extracellular signals to an acute cellular growth response. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.
Recombinant c-Myc protein is suitable for DNA and protein-protein interaction assays. 100 ng are sufficient for DNA-protein and protein-protein interaction studies. The standard curve for TransAM® c-Myc was generated using the range of 5-0.625 ng of protein. The molecular weight of the protein is ~50 kDa. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
Recombinant c-Myc was expressed in a bacterial system (accession number NM 002467) with an N-terminal His-Tag and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 75% homogeneous and contains no detectable protease, DNase and RNase activity.
This product was used in the following publications:
Acta Biochim Pol. 2011;58(4):529-34. Epub 2011 Nov 8.
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.