P19 nuclear extract

Catalog No: 36074 Format: 200 µg $185 Buy

Contents

200 µg of P19 nuclear extract at 5.3 µg/µl.

Background

P19 nuclear extract was prepared from a cell culture of the murine teratocarcinoma P19 cell line. The P19 cell line was originally derived from an experimental embryonic carcinoma induced in a C3H/He strain mouse. Unlike benign teratomas, malignant teratocarcinomas contain populations of undifferentiated stem cells and are characterized by the presence of various differentiated tissues, including teeth, hair and limbs. P19 cells embryonic carcinoma (EC) cells are multipotent and are unique in that they have the ability to change from a malignant to a non-malignant phenotype by the process of cellular differentiation. P19 cells can be induced to differentiate into cell types of all three germ layers. Application of DMSO induces differentiation of P19 embryoid bodies into cardiac and skeletal muscle phenotypes, while retinoic acid treatment induces differentiation into neuronal and glial phenotypes. The P19 cell line is most commonly used in research as a model for cellular differentiation and in studies of physiology and developmental defects.

Application Notes

P19 nuclear extract is recommended for use in studies related to cellular differentiation, physiology and developmental defects.

Extract Origin

Mouse teratocarcinoma

Extract Composition

P19 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Quality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

 

Figure 1: Monitoring Oct-4 activation with the TransAM Oct-4 Kit.
Different amounts of nuclear extracts from unstimulated P19 and HeLa cells are tested for Oct-4 activation using the TransAM Oct-4 Kit.

Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.