Histone H2AS129ph antibody (pAb)

RRID: AB_2793213
Catalog No: 39271 Format: 200 µl $470 Buy
Catalog No: 39272 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
14 kDa
Reactivity:
Budding Yeast, Wide Range Predicted

Applications

ChIP Validated Western Blot Validated Dot Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:2,000 dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Histone H2A phospho Ser129 antibody was raised against a peptide including phospho-serine 129 of budding yeast Histone H2A.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 

Histone H2A phospho Ser129 pAb tested by Western blot.
Whole-cell extract of yeast exposed to 200Gy ionizing radiation blotted with Histone H2A phospho Ser129 pAb at a dilution of 1:1,000.
    Lane 1: Yeast containing an alanine at position 129.
    Lane 2: Wild-type yeast.

Histone H2A phospho Ser129 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2A phospho Ser129 pAb for phospho Ser129 histone H2A. Peptides corresponding to the immunogen and the unmodified version of the immunogen were spotted onto PVDF and probed with the antibody at 1:500. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: Phospho-Ser129 peptide.
    Lane 2: Unmodified Ser129 peptide.

Background

Histone H2A is one of the core components of the nucleosome, the basic building block of chromatin. Unlike mammals that have a distinct DNA damage-responsive histone H2A variant, H2AX, budding yeast histone H2A contains an SQ motif in the C-terminus that is phosphorylated by the Mec1 and Tel1 kinases in response to DNA double strand breaks. As with mammalian H2AX phosphorylation, phosphorylation of yeast histone H2A at serine 129 is required for the efficient repair of DNA double-strand breaks.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H2AS129ph antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting