MTA2 antibody (pAb)

Aliases: PID, MTA1L1
Catalog No: 39359 Format: 200 µl $435 Buy
Catalog No: 39360 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
70 kDa
Reactivity:
Human, Mouse, Other (Wide Range)

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Immunogen

This MTA2 antibody was raised against a peptide corresponding to the C-terminus of human MTA2.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
MTA2 antibody (pAb) tested by Western blot.

MTA2 pAb tested by Western blot.
Nuclear extract (25 µg) of HEK293 (Lane 1) and CSML0 cells (Lane 2) was probed with MTA2 pAb (1:2,000).

Background

MTA2 (Metastasis-associated protein 2) is a member of the Mi-2/NuRD complex, a nucleosome (chromatin) remodeling deacetylase complex. The MTA family sequences contain several common domains, potentially involved in protein-protein interactions (BAH domain), in DNA binding (SANT domain and GATA-type zinc finger motif) and in signal transduction involving many kinase, adaptor and scaffolding proteins (src-homology (SH)-binding motifs). Tumor suppressor p53 protein is deacetylated by both MTA1 and MTA2, leading to inhibition of growth arrest in transforming cells supporting a link between these protein family members and metastasis. MTA2 is ubiquitously expressed.

Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for MTA2 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting