TORC2 antibody (pAb)

Aliases: CRTC2
Catalog No: 39779 Format: 100 µl $435 Buy
Catalog No: 39780 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
85 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 5 - 10 µl per IP
WB: 1:500 - 1:2,000 dilution

Immunogen

This TORC2 antibody was raised against a peptide at the C-terminus of human TORC2.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
TORC2 antibody (pAb) tested by immunoprecipitation.

TORC2 antibody (pAb) tested by Immunoprecipitation.
10 µl of TORC2 antibody was used to immunoprecipitate TORC2 from 400 µg of HEK293 whole cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the TORC2 antibody at a dilution of 1:2,000.

TORC2 antibody (pAb) tested by Western blot.

TORC2 antibody (pAb) tested by Western blot.
Detection of TORC2 by Western blot analysis. Whole cell extract (20 µg) of NIH/3T3 (lane 1) and HEK293 (lane 2) blotted with TORC2 antibody at a dilution of 1:2,000.

Background

TORC2 (Transducer of regulated cAMP response element-binding protein 2) (CRTC2 or CREB-regulated transcription coactivator 2) is a coactivator of transcription through the CREB1 signaling pathway, independent of phosphorylation at serine 133. Involved in metabolic signaling pathways, it is responsive to glucagon signaling and regulates gluconeogenesis. It is a potent activator of mitochondrial biogenesis in muscle cells. TORC2 is inactivated by phosphorylation at serine 171 (in response to both insulin signaling and the activation of AMPK), which facilitates recognition by 14-3-3 protein and subsequent translocation to the cytoplasm.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting