AID antibody (mAb)

RRID: AB_2793380
Clone: 328.8b
Aliases: AICDA, ARP2, CDA2, HIGM2, HEL-S-284
Catalog No: 39885 Format: 100 µg $455 Buy
Catalog No: 39886 Format: 10 µg $110 Buy

Request a quote for a bulk order Bulk Order Button


Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein G Chromatography
Host:
Rat
Molecular Weight:
24 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 3 µg/ml dilution

Published Applications:
ChIP
WB

See references for more information. Individual optimization may be required.

Immunogen

This AID antbody was raised against a peptide corresponding to amino acids 178-198 of human AID.

Buffer

Purified IgG in 50 mM Tris-HCl (pH 7.2), 30% glycerol, and 0.035% sodium azide. Sodium azide is highly toxic.

References

 

AID antibody (mAb) tested by Western blot.
Murine spleen cells were probed with AID antibody at a dilution of 3 µg/ml.

Lane 1: Unstimulated spleen cells from wild type mice.
Lane 2: Treated (LPS & IL4 for 96 hrs) spleen cells from wild type mice.
Lane 3: Unstimulated spleen cells from AID knock-out mice.
Lane 4: Treated (LPS & IL4 for 96 hrs) spleen cells from AID knock-out mice.

Background

AID (Activation-induced cytidine deaminase, AICDA) is a member of the cytidine and deoxycytidylate deaminase family. It acts act as a single-strand DNA deaminase involved in somatic hypermutation, gene conversion and class-switch recombination in developing B-cells and is necessary for efficient antibody responses. It has also been implicated in active DNA demethylation, as deamination of methylcytosine yields thymine, which is read as a mismatch and is replaced by DNA damage repair enzymes.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AID antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting