Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items

ELP3 antibody (pAb)

Aliases: KAT9
Catalog No: 39949 Format: 100 µl $425 Buy Now
Catalog No: 39950 Format: 10 µl $99 Buy Now

Request a quote for a bulk orderBulk Order Button

Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:62 kDa


Western Blot Validated

for ELP3 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:1,000 dilution

Published Applications:

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for ELP3 antibody (pAb)Immunogen

This ELP3 antibody was raised against a peptide within the N-terminal half of human ELP3.

for ELP3 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for ELP3 antibody (pAb)References

ELP3 antibody (pAb) tested by Western blot.

ELP3 antibody (pAb) tested by Western blot.
Nuclear extract of K-562 cells (20 µg) probed with ELP3 antibody (pAb) at a dilution of 1:500.

for ELP3 antibody (pAb)Background

ELP3 (Elongator Protein 3, also called KAT9) was originally identified as a component of the RNA polymerase II elongator complex, involved in transcriptional elongation by RNA Pol II. Elongator, through ELP3 may play a role in chromatin remodeling and is involved in acetylation of histones H3 and possibly H4. ELP3 may also play a role as a DNA demethylase in vertebrates, as it is important for paternal DNA demethylation.

for ELP3 antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for ELP3 antibody.

for ELP3 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for ELP3 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

ELP3 antibody (pAb)

Data Thumbnails

ELP3 antibody (pAb) tested by Western blot.

Western blot of ELP3 pAb.
(Click image to enlarge and see details.)