ELP3 antibody (pAb)
|Catalog No: 39949||Format: 100 µl||$460||Buy|
|Catalog No: 39950||Format: 10 µl||$115||Buy|
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Applications Validated by Active Motif:
WB*: 1:500 - 1:1,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
This ELP3 antibody was raised against a peptide within the N-terminal half of human ELP3.
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
ELP3 (Elongator Protein 3, also called KAT9) was originally identified as a component of the RNA polymerase II elongator complex, involved in transcriptional elongation by RNA Pol II. Elongator, through ELP3 may play a role in chromatin remodeling and is involved in acetylation of histones H3 and possibly H4. ELP3 may also play a role as a DNA demethylase in vertebrates, as it is important for paternal DNA demethylation.
Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for ELP3 antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting