DHX9 antibody (mAb)

Clone: 8E3
Aliases: RHA, Ddx9, HEL-5, NDHII, mHEL-5, AI326842
Catalog No: 61705 Format: 100 µg $435 Buy
Catalog No: 61706 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein A Chromatography
Host:
Rat
Molecular Weight:
150 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 2 - 10 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Immunogen

This antibody was raised against a peptide within the N-terminal region of mouse DHX9.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
DHX9 antibody (mAb) tested by immunofluorescence.

DHX9 antibody (mAb) (Clone 8E3) tested by Immunofluorescence.
Left: Formaldehyde fixed L929 cells stained with DHX9 antibody (mAb). Right: Hoechst.

DHX9 antibody (mAb) tested by Western blot.

DHX9 antibody (mAb) (Clone 8E3) tested by Western blot.
DHX9 antibody detection by Western blot. The analysis was performed using 30 µg of either NIH-3T3 nuclear extract (lane 1), NIH-3T3 cytoplasmic extract (lane 2), or HeLa nuclear extract (lane 3) and DHX9 antibody (mAb) at a 2 µg/ml dilution.

Background

DHX9 (DEAH (Asp-Glu-Ala-His) Box Helicase 9) unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Positively regulates HIV-1 LTR-directed gene expression.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with DHX9 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting